Evidence map›Paper›PMID 38656523›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato.

Valluri V Satyavathi, Kunnappady Princy, Neha Gupta, Narasimha Rao Nizampatnam, Rameshwar Sharma, Yellamaraju Sreelakshmi

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Valluri V SatyavathiRepository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, India.
Kunnappady PrincyRepository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, India.
Neha GuptaRepository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, India.
Narasimha Rao NizampatnamATGC Biotech Pvt. Ltd, Hyderabad, India.
Rameshwar SharmaRepository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, India.
Yellamaraju SreelakshmiRepository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas 9 (CRISPR-associated protein 9) is a robust DNA-encoded, RNA-mediated sequence-specific nuclease system widely used for genome editing of various plants. Although there are many reports on the assembly of gRNAs and plant transformation, there is no single resource for the complete gene editing methodology in tomato. This chapter provides a comprehensive protocol for designing gRNAs, their assembly into the vector, plant transformation, and final mutant analysis in tomato.

Indexed as

CRISPR-Cas SystemsGene EditingGenetic VectorsRNA, Guide, CRISPR-Cas SystemsSolanum lycopersicumGenome, PlantPlants, Genetically ModifiedTransformation, GeneticRNA, Guide, CRISPR-Cas SystemsCRISPR Cas9Multiple gRNAPlant tissue culturePlant transformation

Identifiers

PMID38656523

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.