Evidence map›Paper›PMID 38656427›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2024

Freezing and thawing in Antarctica: characterization of antifreeze protein (AFP) producing microorganisms isolated from King George Island, Antarctica.

J C Lopes, V P Veiga, B Seminiuk, L O F Santos, A M C Luiz, C A Fernandes, C T Kinasz, V H Pellizari, R T D Duarte

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Microbial oil production by Yarrowia lipolytica strains using glycerol as carbon source.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

J C LopesLaboratory of Molecular Ecology and Extremophiles, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n Trindade, 88040-900, Florianópolis, SC, Brazil.
V P VeigaLaboratory of Molecular Ecology and Extremophiles, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n Trindade, 88040-900, Florianópolis, SC, Brazil.
B SeminiukLaboratory of Molecular Ecology and Extremophiles, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n Trindade, 88040-900, Florianópolis, SC, Brazil.
L O F SantosLaboratory of Molecular Ecology and Extremophiles, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n Trindade, 88040-900, Florianópolis, SC, Brazil.
A M C LuizLaboratory of Molecular Ecology and Extremophiles, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n Trindade, 88040-900, Florianópolis, SC, Brazil.
C A FernandesLaboratory of Molecular Ecology and Extremophiles, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n Trindade, 88040-900, Florianópolis, SC, Brazil.
C T KinaszLaboratory of Molecular Ecology and Extremophiles, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n Trindade, 88040-900, Florianópolis, SC, Brazil.
V H PellizariOceanographic Institute, Department of Biological Oceanography, University of São Paulo, 05508-120, São Paulo, SP, Brazil.
R T D DuarteLaboratory of Molecular Ecology and Extremophiles, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n Trindade, 88040-900, Florianópolis, SC, Brazil. rubens.duarte@ufsc.br.ORCID http://orcid.org/0000-0002-9939-3400
Universidade Federal de Santa Catarina · BRInstituto Español de Oceanografía · ES

Funding

Re-Engineering Translational Research at the University of ChicagoUL1TR000430 · NCATS · UNIVERSITY OF CHICAGO · PI SOLWAY, JULIAN · 2012 to 2016
$20.2M
Conselho Nacional de Desenvolvimento Científico e Tecnológico 406850/2022-4Conselho Nacional de Desenvolvimento Científico e Tecnológico 426949/2016-1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88882.438313/2019-01Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887.497695/2020-00Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887.927812/2023-00Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina 2021TR000430NCATS NIH HHS UL1 TR000430
6 · The paper itself

Abstract

Antarctic temperature variations and long periods of freezing shaped the evolution of microorganisms with unique survival mechanisms. These resilient organisms exhibit several adaptations for life in extreme cold. In such ecosystems, microorganisms endure the absence of liquid water and exhibit resistance to freezing by producing water-binding molecules such as antifreeze proteins (AFP). AFPs modify the ice structure, lower the freezing point, and inhibit recrystallization. The objective of this study was to select and identify microorganisms isolated from different Antarctic ecosystems based on their resistance to temperatures below 0 °C. Furthermore, the study sought to characterize these microorganisms regarding their potential antifreeze adaptive mechanisms. Samples of soil, moss, permafrost, and marine sediment were collected on King George Island, located in the South Shetland archipelago, Antarctica. Bacteria and yeasts were isolated and subjected to freezing-resistance and ice recrystallization inhibition (IR) tests. A total of 215 microorganisms were isolated, out of which 118 were molecularly identified through molecular analysis using the 16S rRNA and ITS regions. Furthermore, our study identified 24 freezing-resistant isolates, including two yeasts and 22 bacteria. A total of 131 protein extracts were subjected to the IR test, revealing 14 isolates positive for AFP production. Finally, four isolates showed both freeze-resistance and IR activity (Arthrobacter sp. BGS04, Pseudomonas sp. BGS05, Cryobacterium sp. P64, and Acinetobacter sp. M1_25C). This study emphasizes the diversity of Antarctic microorganisms with the ability to tolerate freezing conditions. These microorganisms warrant further investigation to conduct a comprehensive analysis of their antifreeze capabilities, with the goal of exploring their potential for future biotechnological applications.

Indexed as

Antifreeze ProteinsBacteriaFreezingAntarctic RegionsEcosystemIslandsPhylogenyRNA, Ribosomal, 16SYeastsAntifreeze ProteinsRNA, Ribosomal, 16SAntarcticaAntifreeze proteinsExtremophilesFreezing-resistance

Identifiers

PMID38656427
PMCPMC11153389
OpenAlexW4395077186

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.