ArticleBMC plant biology2024
Control of leaf development in the water fern Ceratopteris richardii by the auxin efflux transporter CrPINMa in the CRISPR/Cas9 analysis.
Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- A Rapid and Highly Effective Protocol for Sporophyte Proliferation ofPlants (Basel, Switzerland) · 2026Article
- Structural Prioritization of FatB Thioesterase Candidates Potentially Related to Lauric Acid-Rich Seed Oil inBiomolecules · 2026Article
- Resolving the Stasis-Dynamism Paradox: Genome Evolution in Tree Ferns.Molecular biology and evolution · 2025Article
- Efficient In Vitro Plantlet Regeneration from Stolon Explants and Genetic Stability Assessment Using ISSR Markers in the Ornamental FernPlants (Basel, Switzerland) · 2025Article
- Spatial regulation of ribosomal protein gene expression revealed by spatial transcriptomic analysis in the water fernFrontiers in plant science · 2025Article
- Efficient gene editing of a model fern species through gametophyte-based transformation.Plant physiology · 2024Article
- Analysis of auxin responses in the fern Ceratopteris richardii identifies the developmental phase as a major determinant for response properties.Development (Cambridge, England) · 2024Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPIN-FORMED genes (PINs) are crucial in plant development as they determine the directionality of auxin flow. They are present in almost all land plants and even in green algae. However, their role in fern development has not yet been determined. This study aims to investigate the function of CrPINMa in the quasi-model water fern Ceratopteris richardii.
resultsCrPINMa possessed a long central hydrophilic loop and characteristic motifs within it, which indicated that it belonged to the canonical rather than the non-canonical PINs. CrPINMa was positioned in the lineage leading to Arabidopsis PIN6 but not that to its PIN1, and it had undergone numerous gene duplications. CRISPR/Cas9 genome editing had been performed in ferns for the first time, producing diverse mutations including local frameshifts for CrPINMa. Plants possessing disrupted CrPINMa exhibited retarded leaf emergence and reduced leaf size though they could survive and reproduce at the same time. CrPINMa transcripts were distributed in the shoot apical meristem, leaf primordia and their vasculature. Finally, CrPINMa proteins were localized to the plasma membrane rather than other cell parts.
conclusionsCRISPR/Cas9 genome editing is feasible in ferns, and that PINs can play a role in fern leaf development.
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