Evidence map›Paper›PMID 38653931›Full record

ArticleCardiovascular engineering and technology2024

Enabling Non-invasive Tracking of Vascular Endothelial Cells Derived from Induced Pluripotent Stem Cells Using Nuclear Imaging.

Jimmy Su, Huifeng Wang, Chad Haney, Guillermo Ameer, Bin Jiang

Abstract read
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In one paragraph

Article in Cardiovascular engineering and technology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Jimmy Su *Center for Advanced Regenerative Engineering, Northwestern University, Evanston, IL, 60208, USA.
Huifeng Wang *Center for Advanced Regenerative Engineering, Northwestern University, Evanston, IL, 60208, USA.
Chad HaneyDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Guillermo AmeerCenter for Advanced Regenerative Engineering, Northwestern University, Evanston, IL, 60208, USA.
Bin JiangCenter for Advanced Regenerative Engineering, Northwestern University, Evanston, IL, 60208, USA. bin.jiang@northwestern.edu.ORCID 0000-0003-2390-4312
Northwestern University · US

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI LEONIDAS C. PLATANIAS · 1993 to 2026
$153.9M
A SPECT_CT Scanner for Preclinical Imaging StudiesS10OD016398 · OD · NORTHWESTERN UNIVERSITY · PI ZHAO, MING · 2014 to 2014
$600k
Enabling Non-Invasive Cell Tracking for Patient-Specific Vascular Endothelial CellsR03EB027998 · NIBIB · NORTHWESTERN UNIVERSITY · PI JIANG, BIN · 2019 to 2020
$158k
NCI NIH HHS P30 CA060553NIBIB NIH HHS 1R03EB027998NIH HHS S10 OD016398
6 · The paper itself

Abstract

purposeThe absence of clinically applicable imaging techniques for continuous monitoring of transplanted cells poses a significant obstacle to the clinical translation of stem cell-based therapies for vascular regeneration. This study aims to optimize a clinically applicable, non-invasive imaging technique to longitudinally monitor vascular endothelial cells (ECs) for vascular regeneration in peripheral artery disease (PAD).

methodsHuman induced pluripotent stem cells (HiPSCs) were employed to generate ECs (HiPSC-ECs). Lentiviral vectors encoding human sodium iodide symporter (hNIS) and enhanced green fluorescent protein (eGFP) genes were introduced to HiPSCs and HiPSC-ECs at varying multiplicities of infection (MOI). Through a combination of fluorescence microscopy and flow cytometry, an optimized transduction technique for introducing hNIS-eGFP into HiPSC-ECs was established. Subsequently, single-photon emission computed tomography (SPECT) was utilized for imaging of the transduced cells in vitro and in vivo after transplantation into the gastrocnemius muscle of nude mice.

resultsLentiviral transduction resulted in sustained co-expression of hNIS and eGFP in HiPSC-ECs when transduced post-endothelial differentiation. An optimal MOI of five yielded over 90% hNIS-eGFP expression efficiency without compromising cell viability. hNIS-eGFP

conclusionsThis study establishes an optimized cell modification and imaging protocol for tracking transplanted cells. Future efforts will focus on enhancing cell survival and integration via improved delivery systems, thereby advancing the potential of cell-based therapies for PAD.

Indexed as

Cell DifferentiationCell TrackingEndothelial CellsGreen Fluorescent ProteinsInduced Pluripotent Stem CellsMice, NudeTomography, Emission-Computed, Single-PhotonAnimalsCell LineCells, CulturedFlow CytometryHumansLentivirusMicroscopy, FluorescenceMuscle, SkeletalPeripheral Arterial Diseaseenhanced green fluorescent proteinGreen Fluorescent ProteinsSodium-Iodide SymportersSymportersEndothelial cellsInduced pluripotent stem cellsNon-invasive imagingSingle-photon emission computed tomographyVascular regeneration

Identifiers

PMID38653931
OpenAlexW4395050992

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.