ArticleProceedings of the National Academy of Sciences of the United States of America1985
Depolarization regulates adrenal preproenkephalin mRNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 76 citations in OpenAlex.
- Regulation of proenkephalin A gene expression in aggregating fetal rat brain cells.Cellular and molecular neurobiology · 1991Article
- Factors regulating the activity of striatal neurons: new perspectives from in situ hybridization histochemistry.Cellular and molecular neurobiology · 1990Review
- Localisation of chromogranin A and B, met-enkephalin-arg6-gly7-leu8 and PGP9.5-like immunoreactivity in the developing and adult rat adrenal medulla and extra-adrenal chromaffin tissue.Journal of anatomy · 1989Article
- Regulation of discrete sub-populations of transmitter-identified neurones after inhibition of electrical activity in cultures of mouse spinal cord.Cell and tissue research · 1989Article
- Prolonged RNA changes in the Hermissenda eye induced by classical conditioning.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Forskolin and phorbol esters decrease the same K+ conductance in cultured oligodendrocytes.The Journal of membrane biology · 1988Article
- Neural and humoral factors separately regulate neuropeptide Y, enkephalin, and chromogranin A and B mRNA levels in rat adrenal medulla.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Electrical stimulation in vivo increases the expression of proenkephalin mRNA and decreases the expression of prodynorphin mRNA in rat hippocampal granule cells.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Regulation of carboxypeptidase H by inhibitory and stimulatory mechanisms during neuropeptide precursor processing.Cellular and molecular neurobiology · 1988Review
- The enkephalin-containing cell: strategies for polypeptide synthesis and secretion throughout the neuroendocrine system.Cellular and molecular neurobiology · 1987Review
- Depolarizing influences regulate preprotachykinin mRNA in sympathetic neurons.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- The synthesis of NPY and DBH is independently regulated in adrenergic nerves after reserpine.Neurochemical research · 1987Article
- Reflex splanchnic nerve stimulation increases levels of proenkephalin A mRNA and proenkephalin A-related peptides in the rat adrenal medulla.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Proenkephalin A gene expression in bovine adrenal chromaffin cells is regulated by changes in electrical activity.The EMBO journal · 1986Article
- Activity-dependent regulation of gene expression in muscle and neuronal cells.Molecular neurobiologyReview
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The regulation of neuropeptide gene expression has been investigated by using rat adrenal medullae grown in explant culture. After 3 days in culture the (now denervated) explants exhibited a 10-fold increase in leucine-enkephalin (leu-EK) content. Inhibition of protein synthesis with cycloheximide completely blocked the rise, whereas inhibition of RNA synthesis with actinomycin D or alpha-amanitin inhibited the increase by 50%. Inhibition of DNA synthesis with 1-beta-D-arabinofuranosylcytosine (cytosine arabinoside) had no discernible effect. To determine whether the rise in leu-EK was associated with an increase in specific mRNA coding for the opiate peptide precursor, blot hybridization analysis was performed. A single species of preproenkephalin mRNA was detected after various culture periods. The amount of mRNA increased 34-fold after 2 days in culture and 74-fold after 4 days. Consequently, the rise in mRNA levels preceded the increase in the amount of leu-EK. Depolarization of the adrenal medullae with either elevated potassium or veratridine, which prevents leu-EK accumulation, inhibited the increase in the amount of preproenkephalin mRNA. Moreover, the effect of veratridine was blocked by tetrodotoxin, suggesting that transmembrane sodium ion influx affects the increase in the amount of message. Our studies suggest that elevation of leu-EK in explanted (denervated) medullae is associated with increased amounts of mRNA coding for the peptide precursor and that these processes can be regulated by depolarization.
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