ArticleBioresources and bioprocessing2021
Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine.
Article in Bioresources and bioprocessing, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Metabolic engineering ofApplied and environmental microbiology · 2026Review
- Methanol-based biosynthesis of p-coumaric acid by engineered Pichia pastoris.Bioresources and bioprocessing · 2026Article
- A random fractionalMagnetic resonance letters · 2026Article
- Recent advances in nonprotein amino acids: insights from function to biosynthesis.Journal of animal science and biotechnology · 2026Review
- Yeast Midas' touch: recent advances in the valorisation of methanol by yeasts.World journal of microbiology & biotechnology · 2025Review
- ADP-ribose is a competitive inhibitor of methanol dehydrogenases from Bacillus methanolicus.The Journal of biological chemistry · 2025Article
- Promoter engineering for enhanced 3-hydroxypropionic acid production inSynthetic and systems biotechnology · 2025Article
- Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol.Journal of biological engineering · 2025Article
- One-Pot Synthesis of β-Alanine from Fumaric Acid via an Efficient Dual-Enzyme Cascade Biotransformation.Biomolecules · 2024Article
- Pathway reconstruction and metabolic engineering for the de novo and enhancing production of monacolin J in Pichia pastoris.Bioprocess and biosystems engineering · 2024Article
- Candida boidinii isolates from olive curation water: a promising platform for methanol-based biomanufacturing.AMB Express · 2024Article
- Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid.Microbial cell factories · 2023Article
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Advances in the synthesis of β-alanine.Frontiers in bioengineering and biotechnology · 2023Review
- Comparative proteomics analysis ofSynthetic and systems biotechnology · 2022Article
- Production of free fatty acids from various carbon sources by Ogataea polymorpha.Bioresources and bioprocessing · 2022Article
- Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.Bioresources and bioprocessing · 2022Article
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3 authors.
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Abstract
β-Alanine (3-aminopropionic acid) is the only naturally occurring β-amino acid and an important precursor for the synthesis of a variety of nitrogen-containing chemicals. Fermentative production of β-alanine from renewable feedstocks such as glucose has attracted significant interest in recent years. Methanol has become an emerging and promising renewable feedstock for biomanufacturing as an alternative to glucose. In this work, we demonstrated the feasibility of β-alanine production from methanol using Pichia pastoris (Komagataella phaffii) as a methylotrophic cell factory. L-Aspartate-α-decarboxylases (ADCs) from different sources were screened and expressed in P. pastoris, followed by the optimization of aspartate decarboxylation by increasing the ADC copy number and C4 precursor supply via the overexpression of aspartate dehydrogenase. The production potential of the best strain was further evaluated in a 1-L fermenter, and a β-alanine titer of 5.6 g/L was obtained. To our best knowledge, this is the highest metabolite production titer ever reached in P. pastoris using methanol as the substrate.
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