Evidence map›Paper›PMID 38650251›Full record

ArticleBioresources and bioprocessing2021

A novel decrystallizing protein CxEXL22 from Arthrobotrys sp. CX1 capable of synergistically hydrolyzing cellulose with cellulases.

Rong Li, Yunze Sun, Yihao Zhou, Jiawei Gai, Linlu You, Fan Yang, Wenzhu Tang, Xianzhen Li

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioresources and bioprocessing, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Rong LiSchool of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, China.
Yunze SunSchool of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, China.
Yihao ZhouSchool of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, China.
Jiawei GaiSchool of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, China.
Linlu YouSchool of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, China.
Fan YangSchool of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, China.
Wenzhu TangSchool of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, China.
Xianzhen LiSchool of Biological Engineering, Dalian Polytechnic University, Gangjingqu, Dalian, 116034, China. xianzhen@mail.com.
Dalian Polytechnic University · CN

Funding

foundation of liaoning province education administration J2019015national natural science foundation of china 31600640Science and technology Department of Dalian 2020RQ118
6 · The paper itself

Abstract

A novel expansin-like protein (CxEXL22) has been identified and characterized from newly isolated Arthrobotrys sp. CX1 that can cause cellulose decrystallization. Unlike previously reported expansin-like proteins from microbes, CxEXL22 has a parallel β-sheet domain at the N terminal, containing many hydrophobic residues to form the hydrophobic surface as part of the groove. The direct phylogenetic relationship implied the genetic transfers occurred from nematode to nematicidal fungal Arthrobotrys sp. CX1. CxEXL22 showed strong activity for the hydrolysis of hydrogen bonds between cellulose molecules, especially when highly crystalline cellulose was used as substrate. The hydrolysis efficiency of Avicel was increased 7.9-fold after pretreating with CxEXL22. The rupture characterization of crystalline region indicated that CxEXL22 strongly binds cellulose and breaks up hydrogen bonds in the crystalline regions of cellulose to split cellulose chains, causing significant depolymerization to expose much more microfibrils and enhances cellulose accessibility.

Indexed as

Cellulose depolymerizationCrystalline regionExpansin-like protein

Identifiers

PMID38650251
PMCPMC10992334
OpenAlexW3202866331

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.