ArticleBioresources and bioprocessing2021
One-pot production of butyl butyrate from glucose using a cognate "diamond-shaped" E. coli consortium.
Article in Bioresources and bioprocessing, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 11 citations in OpenAlex.
- Integrated co-cultivation and subsequent esterification: Harnessing Saccharomyces cerevisiae and Clostridium tyrobutyricum for streamlined ester production.Biotechnology for biofuels and bioproducts · 2025Article
- Microbial cell factory for butyl butyrate production: Knowledges and perspectives.Microbial biotechnology · 2024Review
- Simultaneous fermentation and enzymatic biocatalysis-a useful process option?Biotechnology for biofuels and bioproducts · 2024Review
- Comprehensive evaluation for the one-pot biosynthesis of butyl acetate by using microbial mono- and co-cultures.Biotechnology for biofuels · 2021Article
- Microbial production of butyl butyrate: from single strain to cognate consortium.Bioresources and bioprocessing · 2021Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
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Abstract
Esters are widely used in plastics, textile fibers, and general petrochemicals. Usually, esters are produced via chemical synthesis or enzymatic processes from the corresponding alcohols and acids. However, the fermentative production of esters from alcohols and/or acids has recently also become feasible. Here we report a cognate microbial consortium capable of producing butyl butyrate. This microbial consortium consists of two engineered butyrate- and butanol-producing E. coli strains with nearly identical genetic background. The pathways for the synthesis of butyrate and butanol from butyryl-CoA in the respective E. coli strains, together with a lipase-catalyzed esterification reaction, created a "diamond-shaped" consortium. The concentration of butyrate and butanol in the fermentation vessel could be altered by adjusting the inoculation ratios of each E. coli strain in the consortium. After optimization, the consortium produced 7.2 g/L butyl butyrate with a yield of 0.12 g/g glucose without the exogenous addition of butanol or butyrate. To our best knowledge, this is the highest titer and yield of butyl butyrate produced by E. coli reported to date. This study thus provides a new way for the biotechnological production of esters.
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