Evidence map›Paper›PMID 38649415›Full record

ArticleScientific reports2024

Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing.

Zsófia Lanszki, Md Shafeul Islam, Md Foisal Shikder, Md Jalal Uddin Sarder, Shahneaz Ali Khan, Sharmin Chowdhury, Md Nurul Islam, Zsófia Tauber, Gábor Endre Tóth, Ferenc Jakab and 2 more

Abstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Zsófia LanszkiNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary. lanszkizsofi@gmail.com.
Md Shafeul IslamFaculty of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Md Foisal ShikderDepartment of Medicine and Surgery, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.
Md Jalal Uddin SarderFaculty of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Shahneaz Ali KhanDepartment of Physiology Biochemistry and Pharmacology, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.
Sharmin ChowdhuryDepartment of Pathology and Parasitology, Faculty of Veterinary Medicine, One Health Institute, Rajshahi, Bangladesh.
Md Nurul IslamDepartment of Forest and Wildlife Ecology, Wisconsin Cooperative Wildlife Research Unit, University of Wisconsin-Madison, Madison, USA.
Zsófia TauberNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary.
Gábor Endre TóthNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary.
Ferenc JakabNational Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary.
Gábor Kemenesi *National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Pecs, Hungary.
Sazeda Akter *Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.

Funding

National Research, Development and Innovation Office RRF-2.3.1-21-2022-00010
6 · The paper itself

Abstract

Canine distemper virus (CDV) is a highly contagious virus that affects domestic and wild animals, causing severe illness with high mortality rates. Rapid monitoring and sequencing can provide valuable information about circulating CDV strains, which may foster effective vaccination strategies and the successful integration of these into conservation programs. During two site visits in Bangladesh in 2023, we tested a mobile, deployable genomic surveillance setup to explore the genetic diversity and phylogenetic patterns of locally circulating CDV strains. We collected and analysed 355 oral swab samples from stray dogs in Rajshahi and Chattogram cities, Bangladesh. CDV-specific real-time RT-PCR was performed to screen the samples. Out of the 355 samples, 7.4% (10/135) from Rajshahi city and 0.9% (2/220) from Chattogram city tested positive for CDV. We applied a real-time RT-PCR assay and a pan-genotype CDV-specific amplicon-based Nanopore sequencing technology to obtain the near-completes. Five near-complete genome sequences were generated, with phylogenetic relation to the India-1/Asia-5 lineage previously identified in India. This is the first study to provide genomic data on CDV in Bangladesh and the first demonstration of a mobile laboratory setup as a powerful tool in rapid genomic surveillance and risk assessment for CDV in low resource regions.

Indexed as

DistemperDistemper Virus, CanineNanopore SequencingPhylogenyAnimalsBangladeshDogsGenome, ViralGenotypeReal-Time Polymerase Chain ReactionRNA, ViralRNA, Viral

Identifiers

PMID38649415
PMCPMC11035628

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.