ReviewEuropean journal of microbiology & immunology2024
Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations.
Review in European journal of microbiology & immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
104 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Effects of propolis-modified glass ionomer cement on antimicrobial activity and physico-mechanical properties: a systematic review.Odontology · 2026Pooled it
- Pooled it
- A Comprehensive Meta-Analysis on Antimicrobial Resistance Patterns of the Two MajorTransboundary and emerging diseases · 2025Pooled it
- Article
- Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.Biotechnology and bioengineering · 2026Review
- Biocomposite Microparticles Incorporating Ibuprofen and ZnO Nanoparticles for Controlled Drug Delivery and Antimicrobial Therapy.Polymers · 2026Article
- Evaluation of Antimicrobial Activity, Toxicity, and Stability ofJournal of microbiology and biotechnology · 2026Article
- A MarineMarine drugs · 2026Article
- Callus Induction inPlants (Basel, Switzerland) · 2026Article
- Differential Roles of Ag and ZnO in the Bactericidal Activity of Ag/ZnO-Hydroxyapatite Nanocomposites against Multidrug-Associated Methicillin-Resistant Staphylococcus aureus.Current microbiology · 2026Article
- Nature-inspired biomaterial innovations to counter medical device-associated infections.Biomaterials science · 2026Review
- Potential health aspect of fermented Spirulina product with Lacticaseibacillus rhamnosus.Biotechnology letters · 2026Article
- Exploring the Antitubercular, Antimicrobial Potency, ADME-T Calculations and Docking Studies of 1,2,4-Triazole-Bearing Benzimidazoles.Chemistry & biodiversity · 2026Article
- Article
- Emerging Nanobiochar -Hydrogel Therapeutic Systems: Redox Modulation, Biointerface Interactions, and Critical Gaps in In Vitro Evaluation.Gels (Basel, Switzerland) · 2026Review
- Evaluation of the antibacterial and antifungal activity of 80% methanol crude extract and hexane, chloroform, butanol, and aqueous fractions of Suregada procera against selected microbial strains.BMC complementary medicine and therapies · 2026Article
- Beyond Mueller-Hinton agar: comparative evaluation of agar media for antibiotic susceptibility testing and implications for resource-limited laboratory settings.Microbiology spectrum · 2026Article
- In Vitro Regeneration ofAntioxidants (Basel, Switzerland) · 2026Article
- Potentiation of Penicillin G and Selected β-Lactams with Quercetin Against Multidrug-Resistant Bacteria: Mechanistic Insights, Antibacterial Phytochemicals, and Toxicity Evaluation.International journal of molecular sciences · 2026Article
- Genetic Diversity, Population Structure, Integration of Genome-Wide Association Studies and Machine Learning for Antibacterial Trait Analysis in the Mediterranean Spice Laurel (Plants (Basel, Switzerland) · 2026Article
44 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infectious diseases pose a formidable global challenge, compounded by the emergence of antimicrobial resistance. Consequently, researchers are actively exploring novel antimicrobial compounds as potential solutions. This endeavor underscores the pivotal role of methods employed for screening and evaluating antimicrobial activity-a critical step in discovery and characterization of antimicrobial agents. While traditional techniques such as well-diffusion, disk-diffusion, and broth-dilution are commonly utilized in antimicrobial assays, they may encounter limitations concerning reproducibility and speed. Additionally, a diverse array of antimicrobial assays including cross-streaking, poisoned-food, co-culture, time-kill kinetics, resazurin assay, bioautography, etc., are routinely employed in antimicrobial evaluations. Advanced techniques such as flow-cytometry, impedance analysis, and bioluminescent technique may offer rapid and sensitive results, providing deeper insights into the impact of antimicrobials on cellular integrity. However, their higher cost and limited accessibility in certain laboratory settings may present challenges. This article provides a comprehensive overview of assays designed to characterize antimicrobial activity, elucidating their underlying principles, protocols, advantages, and limitations. The primary objective is to enhance understanding of the methodologies designed for evaluating antimicrobial agents in our relentless battle against infectious diseases. By selecting the appropriate antimicrobial testing method, researchers can discern suitable conditions and streamline the identification of effective antimicrobial agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.