Evidence map›Paper›PMID 38647930›Full record

ArticleBioresources and bioprocessing2024

Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization.

Feifei Guan, Xiaoqian Tian, Ruohan Zhang, Yan Zhang, Ningfeng Wu, Jilu Sun, Honglian Zhang, Tao Tu, Huiying Luo, Bin Yao and 2 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioresources and bioprocessing, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Biochemical analyses of a new GH18 chitinase fromApplied and environmental microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Feifei Guan *Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Xiaoqian Tian *Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Ruohan Zhang *Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Yan ZhangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Ningfeng WuBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Jilu SunCollege of Food Science and Technology, Hebei Agricultural University, Hebei Baoding, 071000, China.
Honglian ZhangInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Tao TuInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Huiying LuoInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Bin YaoInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Jian TianInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. tianjian@caas.cn.
Huoqing HuangInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. huanghuoqing@caas.cn.
Chinese Academy of Agricultural Sciences · CNHebei Agricultural University · CNAgricultural Information Institute · CNBiotechnology Research Institute · CNInstitute of Animal Sciences · CN

Funding

The Agricultural Science and Technology Innovation Program CAAS-ZDRW202304the China Agriculture Research System of MOF and MARA CARS-41the National Key R&D Program of China 2021YFC2103002the National Natural Science Foundation of China 32202720
6 · The paper itself

Abstract

Thermophilic endo-chitinases are essential for production of highly polymerized chitooligosaccharides, which are advantageous for plant immunity, animal nutrition and health. However, thermophilic endo-chitinases are scarce and the transformation from exo- to endo-activity of chitinases is still a challenging problem. In this study, to enhance the endo-activity of the thermophilic chitinase Chi304, we proposed two approaches for rational design based on comprehensive structural and evolutionary analyses. Four effective single-point mutants were identified among 28 designed mutations. The ratio of (GlcNAc)

Indexed as

ChitinChitinaseEndo-activityFunctional oligosaccharidesMutants

Identifiers

PMID38647930
PMCPMC10991111
OpenAlexW4392548500

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.