ArticleBioresources and bioprocessing2023
Enhancement of alkaline protease production in recombinant Bacillus licheniformis by response surface methodology.
Article in Bioresources and bioprocessing, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 35 citations in OpenAlex.
- Screening and identification of significant media components using Plackett-Burman design for enhanced extracellular protease production by Bacillus sp. MTCC 511 under mutagenic stress conditions.Bioresources and bioprocessing · 2026Article
- Microbial Enzyme Production: Critical Bottlenecks and Integrated Engineering Solutions.Journal of basic microbiology · 2026Review
- Precision hydrolysis: tailored yeast processing enzymes for yeast-based products.Applied microbiology and biotechnology · 2026Review
- Optimization of protease production by newly isolated Bacillus sp. from the Red Sea using defatted soybean cake.Scientific reports · 2025Article
- Stable protease from Bacillus licheniformis-MA1 strain: statistical production optimization, kinetic and thermodynamic characterization, and application in silver recovery from used X-ray films.Microbial cell factories · 2025Article
- Isolation of marine bacteria with potential for polyhydroxyalkanoate degradation and optimization for enzyme production.Scientific reports · 2025Article
- Metabolic engineering of Bacillus licheniformis DW2 for ectoine production.World journal of microbiology & biotechnology · 2025Article
- Biotechnology revival:Frontiers in microbiology · 2025Review
- Improving butenyl-spinosyn production inFrontiers in microbiology · 2025Article
- Understanding energy fluctuation during the transition state: The role of AbrB in Bacillus licheniformis.Microbial cell factories · 2024Article
- Advances in recombinant protease production: current state and perspectives.World journal of microbiology & biotechnology · 2024Review
- Article
- Microbial-derived salt-tolerant proteases and their applications in high-salt traditional soybean fermented foods: a review.Bioresources and bioprocessing · 2023Review
- Study on active capacity and detergent application potential of low-temperature alkaline serine protease produced by new strain Exiguobacterium indicum 1.2.3.Bioresources and bioprocessing · 2023Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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Abstract
Alkaline protease is widely used in the food, detergent, and pharmaceutical industries because of its comparatively great hydrolysis ability and alkali tolerance. To improve the ability of the recombinant Bacillus licheniformis to produce alkaline protease, single-factor experiments and response surface methodology (RSM) were utilized to determine and develop optimal culture conditions. The results showed that three factors (corn starch content, soybean meal content, and initial medium pH) had significant effects on alkaline protease production (P < 0.05), as determined through the Plackett‒Burman design. The maximum enzyme activity was observed with an optimal medium composition by central composite design (CCD): corn starch, 92.3 g/L; soybean meal, 35.8 g/L; and initial medium pH, 9.58. Under these optimum conditions, the alkaline protease activity of strain BL10::aprE was 15,435.1 U/mL, 82% higher than that in the initial fermentation medium. To further investigate the application of the optimum fermentation medium, the overexpressed strain BL10::aprE/pHYaprE was cultured using the optimized medium to achieve an enzyme activity of 39,233.6 U/mL. The present study achieved the highest enzyme activity of alkaline protease by B. licheniformis at the shake-flask fermentation level, which has important application value for large-scale production.
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