Evidence map›Paper›PMID 38647916›Full record

ReviewBioresources and bioprocessing2023

Research progress of multi-enzyme complexes based on the design of scaffold protein.

Xiangyi Wang, Yi Jiang, Hongling Liu, Haibo Yuan, Di Huang, Tengfei Wang

Open access · diamondAbstract readReview
In one paragraph

Review in Bioresources and bioprocessing, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
6.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 44 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Xiangyi Wang *State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, Shandong, People's Republic of China.
Yi Jiang *State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, Shandong, People's Republic of China.
Hongling LiuState Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, Shandong, People's Republic of China.
Haibo YuanState Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, Shandong, People's Republic of China.
Di HuangState Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, Shandong, People's Republic of China.
Tengfei WangState Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, Shandong, People's Republic of China. wangtengfeisci@163.com.ORCID http://orcid.org/0000-0003-0735-7259
Qilu University of Technology · CN

Funding

Foundation of Qilu University of Technology of Cultivating Subject for Biology and Biochemistry No. 202003Foundation of Qilu University of Technology of Cultivating Subject for Biology and Biochemistry No. 202007Foundation of Qilu University of Technology of Cultivating Subject for Biology and Biochemistry No. 202018Innovative Research Group Project of the National Natural Science Foundation of China 32001632Key Research and Development Program of Zibo 2021XCYF0085Key Technology Research and Development Program of Shandong 2022CXGC010506Natural Science Foundation of Shandong Province ZR2020QB041Natural Science Foundation of Shandong Province ZR202103030132Natural Science Foundation of Shandong Province ZR2021QB074State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology No. ZZ20200136State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology ZZ20200119State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology ZZ20210114
6 · The paper itself

Abstract

Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This paper reviews the recent progress in the design strategy and application of multi-enzyme complexes. First, the metabolic channels in the multi-enzyme complex have been introduced, and the construction strategies of the multi-enzyme complex emerging in recent years have been summarized. Then, the discovered enzyme cascades related to scaffold proteins are discussed, emphasizing on the influence of the linker on the fusion enzyme (fusion protein) and its possible mechanism. This review is expected to provide a more theoretical basis for the modification of multi-enzyme complexes and broaden their applications in synthetic biology.

Indexed as

Enzyme cascadesFusion proteinLinkerScaffold proteinSubstrate channel

Identifiers

PMID38647916
PMCPMC10992622
OpenAlexW4387878904

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.