ArticleBioresources and bioprocessing2022
Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris.
Article in Bioresources and bioprocessing, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- Collagen-Based Microspheres for Biomedical Applications in Drug Delivery and Tissue Engineering.Biomimetics (Basel, Switzerland) · 2026Review
- Progress in the Expression, Purification, and Characterization of Recombinant Collagen.Bioengineering (Basel, Switzerland) · 2026Review
- Methanol feeding strategies for high-yield production of a collagen-based protein in Komagataella phaffii.Applied microbiology and biotechnology · 2025Article
- Discovering novel type I collagen fragments from Cyprinus carpio supporting bone regeneration.Functional & integrative genomics · 2025Article
- Advances in Molecular Function and Recombinant Expression of Human Collagen.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Innovations and challenges in collagen and gelatin production through precision fermentation.World journal of microbiology & biotechnology · 2025Review
- Identification of a human type XVII collagen fragment with high capacity for maintaining skin health.Synthetic and systems biotechnology · 2024Article
- Diffusion model assisted designing self-assembling collagen mimetic peptides as biocompatible materials.Briefings in bioinformatics · 2024Article
- Review
- Expression, characterization, and application of human-like recombinant gelatin.Bioresources and bioprocessing · 2024Article
- Applications of the Methylotrophic YeastJournal of fungi (Basel, Switzerland) · 2024Review
- Recombinant fibrous protein biomaterials meet skin tissue engineering.Frontiers in bioengineering and biotechnology · 2024Review
- Tissue engineering applications of recombinant human collagen: a review of recent progress.Frontiers in bioengineering and biotechnology · 2024Review
- Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene.Bioresources and bioprocessing · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Collagen is a biofunctional protein that has been widely used in many fields, including but not limited to biomedical, cosmetics and skin care, food, and novel materials. Recombinant collagen has great potential as an alternative to collagen extracted from animals because it avoids the immune response, and the yield and properties are stable. However, challenges remain in the industrial application of recombinant collagen, including improving the expression yield, reducing the cost of purification for industry and expanding applications. In this study, a cloning and recombination method was used to heterologously express the recombinant human-like collagen (RHLC) in Pichia pastoris GS115 using the pPIC9k expression vector. The RHLC expression titre was 2.33 g/L via a 5-L fermenter, and the purification was completed within 48 h and was 98% pure. The characteristics of RHLC were investigated. Furthermore, potential applications for RHLC were explored, such as basal collagen sponge preparation, forming films with chitosan and production of collagen hydrolysed peptides. RHLC has various potential applications due to its triple helical structure, thermostability, good biocompatibility and film-forming ability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.