Evidence map›Paper›PMID 38647808›Full record

ArticleBioresources and bioprocessing2022

Reshaping the binding channel of a novel GH113 family β-mannanase from Paenibacillus cineris (PcMan113) for enhanced activity.

Dengyue Sun, Chao Li, Pengpeng Cui, Jie Zhang, Yaolin Zhou, Mian Wu, Xia Li, Teng-Fei Wang, Zhixiong Zeng, Hui-Min Qin

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Article in Bioresources and bioprocessing, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Dengyue SunState Key Laboratory of Biobased Material and Green Papermaking, College of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250100, People's Republic of China.
Chao LiCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
Pengpeng CuiSchool of Bioengineering, Qilu University of Technology, Shandong Province, Jinan, 250353, People's Republic of China.
Jie ZhangSchool of Bioengineering, Qilu University of Technology, Shandong Province, Jinan, 250353, People's Republic of China.
Yaolin ZhouSchool of Bioengineering, Qilu University of Technology, Shandong Province, Jinan, 250353, People's Republic of China.
Mian WuCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
Xia LiSchool of Bioengineering, Qilu University of Technology, Shandong Province, Jinan, 250353, People's Republic of China.
Teng-Fei WangState Key Laboratory of Biobased Material and Green Papermaking, College of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250100, People's Republic of China.
Zhixiong ZengSchool of Bioengineering, Qilu University of Technology, Shandong Province, Jinan, 250353, People's Republic of China. zengzx@qlu.edu.cn.
Hui-Min QinState Key Laboratory of Biobased Material and Green Papermaking, College of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250100, People's Republic of China. huiminqin@tust.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endo-β-mannanases are important enzymes for degrading lignocellulosic biomass to generate mannan, which has significant health effects as a prebiotic that promotes the development of gut microbiota. Here, a novel endo-β-mannanase belonging to glycoside hydrolase (GH) family 113 from Paenibacillus cineris (PcMan113) was cloned, expressed and characterized, as one of only a few reported GH113 family β-mannanases. Compared to other functionally and structurally characterized GH113 mannanases, recombinant PcMan113 showed a broader substrate spectrum and a better performance. Based on a structural homology model, the highly active mutant PcMT3 (F110E/N246Y) was obtained, with 4.60- and 5.53-fold increases of enzyme activity (towards KG) and catalytic efficiency (k

Indexed as

Binding channelEndo-β-mannanaseGH113 familyMolecular dynamicsStructural analysis

Identifiers

PMID38647808
PMCPMC10992819

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.