ArticleBioresources and bioprocessing2022
Selective biosynthesis of retinol in S. cerevisiae.
Article in Bioresources and bioprocessing, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- De novo coassembly of functional chlorophyll- and carotenoid-protein complexes inScience advances · 2026Article
- Metabolic Engineering of Komagataella Phaffii for de Novo Synthesis of Retinol From Methanol.Biotechnology journal · 2026Article
- Efficient vitamin A production in Lipomyces starkeyi through metabolic engineering of the β-carotene and retinoid pathways.Microbial cell factories · 2026Article
- Engineering Modification for the Efficient Synthesis of β-ionone by Yarrowia lipolytica.Biotechnology journal · 2026Article
- Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2026Article
- Multiplex metabolic engineering of yeast for high-efficiency all-trans-retinoic acid production.Microbial cell factories · 2025Article
- Enhanced vitamin A production by engineering transporters, ATP and precursor supply.Biodesign research · 2025Article
- Biosynthesis of Edible Terpenoids: Hosts and Applications.Foods (Basel, Switzerland) · 2025Review
- De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid.Nature communications · 2025Article
- Systematic metabolic engineering enables highly efficient production of vitamin A inSynthetic and systems biotechnology · 2025Article
- Combined metabolic engineering and lipid droplets degradation to increase vitamin A production in Saccharomyces cerevisiae.Microbial cell factories · 2024Article
- Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.Molecules (Basel, Switzerland) · 2024Review
- Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Article
- Sustainable biosynthesis of valuable diterpenes in microbes.Engineering microbiology · 2023Review
- First report on metagenomics and their predictive functional analysis of fermented bamboo shoot food of Tripura, North East India.Frontiers in microbiology · 2023Article
- Molecular Properties of β-Carotene Oxygenases and Their Potential in Industrial Production of Vitamin A and Its Derivatives.Antioxidants (Basel, Switzerland) · 2022Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The vitamin A component retinol has become an increasingly sought-after cosmetic ingredient. In previous efforts for microbial biosynthesis of vitamin A, a mixture of retinoids was produced. In order to efficiently produce retinol at high purity, the precursor and NADPH supply was first enhanced to improve retinoids accumulation in the S. cerevisiae strain constructed from a β-carotene producer by introducing β-carotene 15,15'-dioxygenase, following by screening of heterologous and endogenous oxidoreductases for retinal reduction. Env9 was found as an endogenous retinal reductase and its activity was verified in vitro. By co-expressing Env9 with the E. coli ybbO, as much as 443.43 mg/L of retinol was produced at 98.76% purity in bi-phasic shake-flask culture when the antioxidant butylated hydroxytoluene was added to prevent retinoids degradation. The retinol titer reached 2479.34 mg/L in fed-batch fermentation. The success in selective biosynthesis of retinol would lay a solid foundation for its biotechnological production.
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