ArticleBioresources and bioprocessing2023
Functional tailoring of a PET hydrolytic enzyme expressed in Pichia pastoris.
Article in Bioresources and bioprocessing, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Application of the YebF secretion pathway inRSC chemical biology · 2026Article
- Changes in Mechanical Properties and Structure of PET Films Treated with Metagenome-Derived LCCPolymers · 2026Article
- Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes inBioengineering (Basel, Switzerland) · 2025Review
- Efficient biodegradation and upcycling of polyethylene terephthalate mediated by cell-factories.Frontiers in microbiology · 2025Review
- Engineered polyethylene terephthalate hydrolases: perspectives and limits.Applied microbiology and biotechnology · 2024Review
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Authors and funding
9 authors.
Funding
Abstract
Using enzymes to hydrolyze and recycle poly(ethylene terephthalate) (PET) is an attractive eco-friendly approach to manage the ever-increasing PET wastes, while one major challenge to realize the commercial application of enzyme-based PET degradation is to establish large-scale production methods to produce PET hydrolytic enzyme. To achieve this goal, we exploited the industrial strain Pichia pastoris to express a PET hydrolytic enzyme from Caldimonas taiwanensis termed CtPL-DM. In contrast to the protein expressed in Escherichia coli, CtPL-DM expressed in P. pastoris is inactive in PET degradation. Structural analysis indicates that a putative N-glycosylation site N181 could restrain the conformational change of a substrate-binding Trp and hamper the enzyme action. We thus constructed N181A to remove the N-glycosylation and found that the PET hydrolytic activity of this variant was restored. The performance of N181A was further enhanced via molecular engineering. These results are of valuable in terms of PET hydrolytic enzyme production in industrial strains in the future.
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