ArticleBioresources and bioprocessing2022
Bioproduction of testosterone from phytosterol by Mycolicibacterium neoaurum strains: "one-pot", two modes.
Article in Bioresources and bioprocessing, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Association of lignan metabolites with prevalent stroke and mortality in stroke patients: results from NHANES.Journal of clinical biochemistry and nutrition · 2026Article
- Direct Conversion of Phytosterol to Testosterone via an Integrated Microbial-Enzymatic Strategy.Chemistry & biodiversity · 2026Article
- Toward Sustainable Testosterone Manufacturing: Green Chemistry and Microbial Biotransformation Approaches.International journal of molecular sciences · 2026Review
- Microbial Synthesis and Biological Activity of 20β-Hydroxylated Progestins: Ovarian and Neural Action of 17α,20β,21α-Trihydroxy-4-Pregnen-3-One inBiomolecules · 2026Article
- Pregnenolone Bioproduction in Engineered Methylobacteria: Design and Elaboration.International journal of molecular sciences · 2025Article
- Steroid Hydroxylation by Mutant Cytochrome P450 BM3-LG23 Using Two Expression Chassis.International journal of molecular sciences · 2025Article
- Boldenone and Testosterone Production from Phytosterol via One-Pot Cascade Biotransformations.Journal of fungi (Basel, Switzerland) · 2024Article
- From accurate genome sequence to biotechnological application: The thermophile Mycolicibacterium hassiacum as experimental model.Microbial biotechnology · 2024Article
- Reconstruction of the Steroid 1(2)-Dehydrogenation System fromMicroorganisms · 2023Article
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6 authors.
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Abstract
The main male hormone, testosterone is obtained from cheap and readily available phytosterol using the strains of Mycolicibacterium neoaurum VKM Ac-1815D, or Ac-1816D. During the first "oxidative" stage, phytosterol (5-10 g/L) was aerobically converted by Ac-1815D, or Ac-1816D to form 17-ketoandrostanes: androstenedione, or androstadienedione, respectively. At the same bioreactor, the 17-ketoandrostanes were further transformed to testosterone due to the presence of 17β-hydroxysteroid dehydrogenase activity in the strains ("reductive" mode). The conditions favorable for "oxidative" and "reductive" stages have been revealed to increase the final testosterone yield. Glucose supplement and microaerophilic conditions during the "reductive" mode ensured increased testosterone production by mycolicibacteria cells. Both strains effectively produced testosterone from phytosterol, but highest ever reported testosterone yield was achieved using M. neoaurum VKM Ac-1815D: 4.59 g/l testosterone was reached from 10 g/l phytosterol thus corresponding to the molar yield of over 66%. The results contribute to the knowledge on phytosterol bioconversion by mycolicibacteria, and are of significance for one-pot testosterone bioproduction from phytosterol bypassing the intermediate isolation of the 17-ketoandrostanes.
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