Evidence map›Paper›PMID 38647340›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2024

Neural Tracing Protein-Functionalized Nanoparticles Capable of Fast Retrograde Axonal Transport in Live Neurons.

Wenqian Wang, Md Musfizur Hassan, Natasha Kapoor-Kaushik, Lital Livni, Benjamin Musrie, Jianbo Tang, Zaheri Mahmud, Saluo Lai, Peter Richard Wich, Vaishnavi Ananthanarayanan and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Visualising sub-second dynamics of nanoparticle extravasationbioRxiv : the preprint server for biology · 2025
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Wenqian WangSchool of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.ORCID 0000-0001-6428-6026
Md Musfizur HassanSchool of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
Natasha Kapoor-KaushikElectron Microscopy Unit, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
Lital LivniSchool of Biomedical Sciences, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
Benjamin MusrieSchool of Biomedical Sciences, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
Jianbo TangSchool of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.ORCID 0000-0002-0155-6807
Zaheri MahmudSchool of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
Saluo LaiSchool of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
Peter Richard WichSchool of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.ORCID 0000-0003-1522-0340
Vaishnavi AnanthanarayananEMBL Australia Node in Single Molecule Science, Department of Molecular Medicine, School of Biomedical Sciences, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
Gila Moalem-TaylorSchool of Biomedical Sciences, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.ORCID 0000-0003-3828-9527
Guangzhao MaoSchool of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.ORCID 0000-0001-9308-3922
UNSW Sydney · AU

Funding

Targeted Drug Delivery for Spinal Cord Injury Using Retrograde Transport of a NanoconjugateR61NS112443 · NINDS · WAYNE STATE UNIVERSITY · PI MAO, GUANGZHAO, SANKARI, ABDULGHANI · 2019 to 2020
$684k
ARC Discovery Early Career Researcher Award DE220100816Australian Research Council DP230102641EMBL AustraliaMultiple Sclerosis Australia 22-2-108NIH HHS R61NS112443NINDS NIH HHS R61 NS112443
6 · The paper itself

Abstract

Neural tracing proteins like horseradish peroxidase-conjugated wheat germ agglutinin (WGA-HRP) can target the central nervous system (CNS) through anatomic retrograde transport without crossing the blood-brain barrier (BBB). Conjugating WGA-HRP to nanoparticles may enable the creation of BBB-bypassing nanomedicine. Microfluidics and two-photon confocal microscopy is applied to screen nanocarriers for transport efficacy and gain mechanistic insights into their interactions with neurons. Protein modification of gold nanoparticles alters their cellular uptake at the axonal terminal and activates fast retrograde transport. Trajectory analysis of individual endosomes carrying the nanoparticles reveals a run-and-pause pattern along the axon with endosomes carrying WGA-HRP-conjugated gold nanoparticles exhibiting longer run duration and faster instantaneous velocity than those carrying nonconjugated nanoparticles. The results offer a mechanistic explanation of the different axonal transport dynamics as well as a cell-based functional assay of neuron-targeted nanoparticles with the goal of developing BBB-bypassing nanomedicine for the treatment of nervous system disorders.

Indexed as

Axonal TransportGoldNeuronsAnimalsAxonsMetal NanoparticlesNanoparticlesRatsWheat Germ AgglutininsGoldWheat Germ Agglutininsaxonal transportmicrofluidicsneural tracing proteinsingle‐particle trackingtwo‐photon confocal microscopy

Identifiers

PMID38647340
PMCPMC11427170
OpenAlexW4394999909

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.