Evidence map›Paper›PMID 38646484›Full record

ArticlePeerJ2024

Novel monoclonal antibodies against house dust mite allergen Der p 21 and their application to analyze allergen extracts.

Vytautas Rudokas, Laimis Silimavicius, Indre Kucinskaite-Kodze, Aiste Sliziene, Milda Pleckaityte, Aurelija Zvirbliene

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Vytautas RudokasInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
Laimis SilimaviciusInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
Indre Kucinskaite-KodzeInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
Aiste SlizieneInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
Milda PleckaityteInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
Aurelija ZvirblieneInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
Vilnius University · LT

Funding

European Regional Development FundResearch Council of Lithuania (LMTLT)
6 · The paper itself

Abstract

Background: Allergen extracts and recombinant allergens are used in allergy diagnostics and immunotherapy. Since allergen extracts from different manufacturers lack proper standardization regarding their composition, monoclonal antibodies (MAbs) against specific allergen components can be used for their identification and quantification in allergen extracts. This study aimed to generate MAbs against allergen Der p 21 of Methods: Recombinant Der p 21 was expressed in Results: MAbs raised against recombinant Der p 21 were characterized in detail and proven to be reactive with natural Der p 21. Highly specific sandwich enzyme-linked immunosorbent assay for the quantification of Der p 21 was developed and optimized. The allergen was detected and its concentration was determined in only three of six analyzed HDM allergen extracts from different manufacturers. Conclusion: HDM analysis by MAb-based immunoassays shows their differences in allergen composition. The results demonstrate the importance of allergen-specific MAbs as a tool for the characterization of allergen extracts and the need for their appropriate standardization before their use for allergy diagnostics or immunotherapy.

Indexed as

Antibodies, MonoclonalAntigens, DermatophagoidesEnzyme-Linked Immunosorbent AssayRecombinant ProteinsAllergensAnimalsArthropod ProteinsBlotting, WesternMiceMice, Inbred BALB CPyroglyphidaeAllergensAntibodies, MonoclonalAntigens, DermatophagoidesArthropod ProteinsRecombinant ProteinsAllergen extractDermatophagoides pteronyssinusDer p 21House dust miteMonoclonal antibodies

Identifiers

PMID38646484
PMCPMC11032652
OpenAlexW4394925769

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.