Evidence map›Paper›PMID 38645159›Full record

ArticlebioRxiv : the preprint server for biology2024

Ultrasensitive amplification-free quantification of a methyl CpG-rich cancer biomarker by single-molecule kinetic fingerprinting.

Liuhan Dai, Alexander Johnson-Buck, Peter W Laird, Muneesh Tewari, Nils G Walter

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Liuhan DaiSingle Molecule Analysis Group, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-9742-236X
Alexander Johnson-BuckSingle Molecule Analysis Group, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0003-3748-6000
Peter W LairdDepartment of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA.ORCID 0000-0001-9117-3641
Muneesh TewariCenter for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-7781-3152
Nils G WalterSingle Molecule Analysis Group, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-7301-1275
University of Michigan · USVan Andel Institute · US

Funding

Highly specific, amplification-free, single-molecule counting of rare methylated DNA cancer biomarkersR21CA225493 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TEWARI, MUNEESH, WALTER, NILS G · 2020 to 2020
$609k
NCI NIH HHS R21 CA225493
6 · The paper itself

Abstract

The most well-studied epigenetic marker in humans is the 5-methyl modification of cytosine in DNA, which has great potential as a disease biomarker in liquid biopsies of cell-free DNA. Currently, quantification of DNA methylation relies heavily on bisulfite conversion followed by PCR amplification and NGS or microarray analysis. PCR is subject to potential bias in differential amplification of bisulfite-converted methylated

Identifiers

PMID38645159
PMCPMC11030368
OpenAlexW4394675279

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.