ArticleVeterinary research communications2024
Molecular investigation and genetic characterization of feline leukemia virus (FeLV) in cats referred to a veterinary teaching hospital in Northern Italy.
Article in Veterinary research communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- Cross-species transmission of host adaptation of feline leukemia virus between domestic cats and the wild felid Leopardus guigna.Veterinary research · 2026Article
- Description of a Virulent Systemic Feline Calicivirus Infection in a Kitten with Footpads Oedema and Fatal Pneumonia.Pathogens (Basel, Switzerland) · 2025Article
- Retrospective Analysis of Feline Leukemia Virus (FeLV) Frequency in Domestic Cats in Quito, Ecuador (2021-2024).Animals : an open access journal from MDPI · 2025Article
- Prevalence and genetic characterization of feline leukemia virus in portuguese stray cats.BMC veterinary research · 2025Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Feline leukemia virus (FeLV) is responsible for feline leukemia syndrome in domestic cats. The prevention and control of disease caused by FeLV are primarily based on vaccination and identification and isolation of infected subjects. Antigen diagnostic methods, which are the most widely used in clinical practices, can be associated to molecular tests to characterize the FeLV detected. In this study, a quantitative SYBR Green Real-Time PCR (qPCR) assay was used to detect FeLV proviral DNA in blood samples from antigen positive cats referred to a veterinary teaching hospital in Northern Italy in 2018-2021. To genetically characterize the identified viruses, a portion of the viral envelope (env) gene was amplified using six different end-point PCRs and sequenced. Twenty-two of 26 (84.6%) cats included in the study tested positive by qPCR assay. This suggests a high performance of the qPCR adopted but further studies are required to investigate the cause of discordant results between the antigen test and qPCR in four cats. From env gene analysis, 15/22 qPCR-positive cats were infected by FeLV subtype A and 5/15 shown coinfection with subtype B.
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