Evidence map›Paper›PMID 38643292›Full record

ArticleScientific reports2024

A high-precision wound healing assay based on photosensitized culture substrates.

Saphia Azzam, Lea Tomasova, Carina Danner, Michael Skiba, Maren Klein, Zeno Guttenberg, Stefanie Michaelis, Joachim Wegener

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Saphia Azzam *Institut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany.
Lea Tomasova *Ibidi GmbH, Lochhamer Schlag 11, 82166, Graefelfing, Germany.
Carina DannerInstitut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany.
Michael SkibaInstitut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany.
Maren KleinInstitut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany.
Zeno GuttenbergIbidi GmbH, Lochhamer Schlag 11, 82166, Graefelfing, Germany.
Stefanie MichaelisFraunhofer-Institut Fuer Elektronische Mikrosysteme Und Festkoerper-Technologien EMFT, Universitaetsstr. 31, 93053, Regensburg, Germany.
Joachim WegenerInstitut Fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany. Joachim.Wegener@ur.de.
University of Regensburg · DEFraunhofer Research Institution for Microsystems and Solid State Technologies · DE

Funding

Federal Ministry of Education and Research (BMBF) 13XP5074
6 · The paper itself

Abstract

Quantitative assessment of cell migration in vitro is often required in fundamental and applied research from different biomedical areas including wound repair, tumor metastasis or developmental biology. A collection of assays has been established throughout the years like the most widely used scratch assay or the so-called barrier assay. It is the principle of these assays to introduce a lesion into an otherwise confluent monolayer in order to study the migration of cells from the periphery into this artificial wound and determine the migration rate from the time necessary for wound closure. A novel assay makes use of photosensitizers doped into a polystyrene matrix. A thin layer of this composite material is coated on the bottom of regular cell culture ware showing perfect biocompatibility. When adherent cells are grown on this coating, resonant excitation of the photosensitizer induces a very local generation of

Indexed as

Photosensitizing AgentsWound HealingCell Culture TechniquesCell MovementMicrofluidicsPhotosensitizing AgentsCell migrationMetastasisPhotosensitizerPhototoxicityReactive oxygen speciesSinglet oxygenWound healing

Identifiers

PMID38643292
PMCPMC11032384
OpenAlexW4394988851

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.