Evidence map›Paper›PMID 38641575›Full record

ArticleMolecular medicine (Cambridge, Mass.)2024

F127-SE-tLAP thermosensitive hydrogel alleviates bleomycin-induced skin fibrosis via TGF-β/Smad pathway.

Zhiqin Cao, Keke Zhang, Jingruo Liu, Yu Pan, Jiayi Shi, Luxin Li, Xiaocan Sun, Shiqi Li, Xiaohuan Yuan, Dan Wu

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine (Cambridge, Mass.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Zhiqin CaoHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Keke ZhangHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Jingruo LiuHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Yu PanHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Jiayi ShiHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Luxin LiHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Xiaocan SunHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Shiqi LiHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Xiaohuan YuanHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China.
Dan WuHeilongjiang Province Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, 157011, Mudanjiang, Heilongjiang, China. wudan@mdjmu.edu.cn.ORCID 0000-0002-0241-4691
Mudanjiang Medical University · CN

Funding

Doctoral Research Foundation of Mudanjiang Medical University 2021-MYB-SKY-066Natural Science Foundation of Heilongjiang Province LH2023H053Torch Program Youth Top Talent Project of Mudanjiang Medical University 2022-MYHJ-015
6 · The paper itself

Abstract

backgroundSkin fibrosis affects the normal function of the skin. TGF-β1 is a key cytokine that affects organ fibrosis. The latency-associated peptide (LAP) is essential for TGF-β1 activation. We previously constructed and prepared truncated LAP (tLAP), and confirmed that tLAP inhibited liver fibrosis by affecting TGF-β1. SPACE peptide has both transdermal and transmembrane functions. SPACE promotes the delivery of macromolecules through the stratum corneum into the dermis. This study aimed to alleviate skin fibrosis through the delivery of tLAP by SPACE.

methodsThe SPACE-tLAP (SE-tLAP) recombinant plasmid was constructed. SE-tLAP was purified by nickel affinity chromatography. The effects of SE-tLAP on the proliferation, migration, and expression of fibrosis-related and inflammatory factors were evaluated in TGF-β1-induced NIH-3T3 cells. F127-SE-tLAP hydrogel was constructed by using F127 as a carrier to load SE-tLAP polypeptide. The degradation, drug release, and biocompatibility of F127-SE-tLAP were evaluated. Bleomycin was used to induce skin fibrosis in mice. HE, Masson, and immunohistochemistry were used to observe the skin histological characteristics.

resultsSE-tLAP inhibited the proliferation, migration, and expression of fibrosis-related and inflammatory factors in NIH-3T3 cells. F127-SE-tLAP significantly reduced ECM production, collagen deposition, and fibrotic pathological changes, thereby alleviating skin fibrosis.

conclusionF127-SE-tLAP could increase the transdermal delivery of LAP, reduce the production and deposition of ECM, inhibit the formation of dermal collagen fibers, and alleviate the progression of skin fibrosis. It may provide a new idea for the therapy of skin fibrosis.

Indexed as

PolyethylenesPolypropylenesSkin DiseasesTransforming Growth Factor betaAnimalsBleomycinCollagenFibrosisHydrogelsMiceSignal TransductionSkinSmad ProteinsTransforming Growth Factor beta1BleomycinCollagenHydrogelsPolyethylenesPolypropylenesSmad ProteinsTransforming Growth Factor betaTransforming Growth Factor beta1UCON 50-HB-5100EMTLAPSkin fibrosisSPACETGF-β1

Identifiers

PMID38641575
PMCPMC11031956
OpenAlexW4394963187

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.