Evidence map›Paper›PMID 38640794›Full record

ReviewJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2024

Applications of chromatographic methods in metabolomics: A review.

Susan T Ovbude, Sadia Sharmeen, Isaac Kyei, Harshana Olupathage, Jacob Jones, Richard J Bell, Robert Powers, David S Hage

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Review
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  8. PeakClimber: A software tool for the accurate quantification of complex HPLC chromatograms.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2025
    Article
  9. Article
  10. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Susan T OvbudeDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Sadia SharmeenDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Isaac KyeiDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Harshana OlupathageDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Jacob JonesDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Richard J BellDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Robert PowersDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA; Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
David S HageDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA. Electronic address: dhage1@unl.edu.

Funding

Targeted mass spectrometry approaches to understand CART processing and recepter interactionsP20GM113126 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI GUO, JIANTAO · 2016 to 2025
$20.8M
Redox Biology CenterP30GM103335 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI BECKER, DONALD F · 2012 to 2016
$4.3M
NIGMS NIH HHS P20 GM113126NIGMS NIH HHS P30 GM103335
6 · The paper itself

Abstract

Chromatography is a robust and reliable separation method that can use various stationary phases to separate complex mixtures commonly seen in metabolomics. This review examines the types of chromatography and stationary phases that have been used in targeted or untargeted metabolomics with methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. General considerations for sample pretreatment and separations in metabolomics are considered, along with the various supports and separation formats for chromatography that have been used in such work. The types of liquid chromatography (LC) that have been most extensively used in metabolomics will be examined, such as reversed-phase liquid chromatography and hydrophilic liquid interaction chromatography. In addition, other forms of LC that have been used in more limited applications for metabolomics (e.g., ion-exchange, size-exclusion, and affinity methods) will be discussed to illustrate how these techniques may be utilized for new and future research in this field. Multidimensional LC methods are also discussed, as well as the use of gas chromatography and supercritical fluid chromatography in metabolomics. In addition, the roles of chromatography in NMR- vs. MS-based metabolomics are considered. Applications are given within the field of metabolomics for each type of chromatography, along with potential advantages or limitations of these separation methods.

Indexed as

ChromatographyMetabolomicsAnimalsChromatography, LiquidHumansHydrophobic and Hydrophilic InteractionsMagnetic Resonance SpectroscopyMass SpectrometryGas chromatographyLiquid chromatographyMass spectrometryMetabolomicsNuclear magnetic resonance spectroscopySupercritical fluid chromatography

Identifiers

PMID38640794
PMCPMC11618781

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.