Evidence map›Paper›PMID 38639895›Full record

ArticleFish physiology and biochemistry2024

Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla).

Marta Blanes-García, Zoran Marinović, Juan Germán Herranz-Jusdado, Xuan Xie, Leonor Ferrão, Victor Gallego, Luz Pérez, Abdul Rasheed Baloch, Ákos Horváth, Martin Pšenička and 2 more

Open access · hybridAbstract read
In one paragraph

Article in Fish physiology and biochemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 3 countries.

Marta Blanes-GarcíaGrupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera S/N, 46022, Valencia, Spain.ORCID http://orcid.org/0000-0002-0858-0076
Zoran MarinovićDepartment of Aquaculture, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly U. 1, 2100, Gödöllő, Hungary.ORCID http://orcid.org/0000-0003-3980-8325
Juan Germán Herranz-JusdadoGrupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera S/N, 46022, Valencia, Spain.ORCID http://orcid.org/0000-0001-8773-4637
Xuan XieFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
Leonor FerrãoGrupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera S/N, 46022, Valencia, Spain.
Victor GallegoGrupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera S/N, 46022, Valencia, Spain.
Luz PérezGrupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera S/N, 46022, Valencia, Spain.ORCID http://orcid.org/0000-0002-6678-7446
Abdul Rasheed BalochFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
Ákos HorváthDepartment of Aquaculture, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly U. 1, 2100, Gödöllő, Hungary.ORCID http://orcid.org/0000-0001-9760-2478
Martin PšeničkaFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.ORCID http://orcid.org/0000-0002-3808-7856
Juan F AsturianoGrupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera S/N, 46022, Valencia, Spain. jfastu@dca.upv.es.ORCID http://orcid.org/0000-0002-6441-5294
Marina MoriniGrupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera S/N, 46022, Valencia, Spain.ORCID http://orcid.org/0000-0003-3313-3419
Universitat Politècnica de València · ESUniversity of South Bohemia in České Budějovice · CZMagyar Agrár- és Élettudományi Egyetem · HU

Funding

Biodiversity CZ.02.1.01/0.0/0.0/16_025/0007370Czech Science Foundation 22-31141JEuropean Union NextGenerationEU RYC2021-031558-IEuropean Union NextGenerationEU THINKINAZUL/2021/012European Union's Horizon 2020 Marie Skłodowska-Curie Nº 642893 (IMPRESS)Generalitat Valenciana GRISOLIAP/2020/063Generalitat Valenciana THINKINAZUL/2021/012Ministerio de Ciencia e Innovación RYC2021-031558-IMinisterio de Ciencia e Innovación y Universidades Project EELGONIA; RTI2018-096413-B-I00Ministry of Education, Youth and Sports of the Czech Republic Project CENAKVA (LM2018099)Ministry of Innovation and Technology Hungary TKP2020-NKA-16Universitat Politècnica de València PAID-01-20
6 · The paper itself

Abstract

Identification of specific molecular markers for spermatogonial stem cells in teleost is crucial for enhancing the efficacy of reproductive biotechnologies in aquaculture, such as transplantation and surrogate production in fishes. Since it is not yet possible to distinguish spermatogonial stem cells of European eel (Anguilla anguilla) using specific molecular markers, we isolated spermatogonial cells from immature European eels to find these potential markers. We attempted this by studying three candidate genes: vasa, nanos2, and dnd1. Two vasa (vasa1 and vasa2) genes, nanos2, and dnd1 were identified, characterized, and studied in the muscle, testis, and isolated spermatogonia. Our results showed that vasa1 and vasa2 had the highest levels of expression when measured by qPCR. In situ hybridization and immunochemistry assays showed that the four genes were localized explicitly in type A spermatogonia. However, vasa1 and vasa2 exhibited stronger signals in the immature testicular tissue than the other two potential markers. According to this, vasa1 and vasa2 were found to be the most effective markers for spermatogonial cells in the European eel.

Indexed as

AnguillaBiomarkersSpermatogoniaTestisAnimalsFish ProteinsMaleRNA-Binding ProteinsBiomarkersFish ProteinsRNA-Binding ProteinsDnd1FishGene expressionNanos2TestisVasa

Identifiers

PMID38639895
PMCPMC11576858
OpenAlexW4394962372

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.