Evidence map›Paper›PMID 38637828›Full record

ArticleMalaria journal2024

Aspecific binding of anti-NK1.1 antibodies on myeloid cells in an experimental model for malaria-associated acute respiratory distress syndrome.

Emilie Pollenus, Fran Prenen, Hendrik Possemiers, Sofie Knoops, Tania Mitera, Jochen Lamote, Amber De Visscher, Leen Vandermosten, Thao-Thy Pham, Patrick Matthys and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Emilie PollenusLaboratory of Immunoparasitology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-2706-6259
Fran PrenenLaboratory of Immunoparasitology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-5431-1715
Hendrik PossemiersLaboratory of Immunoparasitology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.
Sofie KnoopsLaboratory of Immunoparasitology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.
Tania MiteraLaboratory of Immunobiology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.
Jochen LamoteLaboratory for Molecular Cancer Biology, Department of Oncology, VIB, KU Leuven, Leuven, Belgium.
Amber De VisscherLaboratory of Immunobiology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-9577-2390
Leen VandermostenLaboratory of Immunoparasitology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.
Thao-Thy PhamLaboratory of Immunoparasitology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0003-3728-6455
Patrick MatthysLaboratory of Immunobiology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-9685-6836
Philippe E Van den SteenLaboratory of Immunoparasitology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium. philippe.vandensteen@kuleuven.be.ORCID http://orcid.org/0000-0002-7334-9145
Rega Institute for Medical Research · BEVIB-KU Leuven Center for Cancer Biology · BE

Funding

Fonds Wetenschappelijk Onderzoek G0C9720NFonds Wetenschappelijk Onderzoek PhD fellowshipL'Oréal-UNESCO For women in Science & Fonds Wetenschappelijk Onderzoek PhD fellowshipResearch Fund of the KU Leuven C1 project C16/17/010
6 · The paper itself

Abstract

backgroundConventional natural killer (cNK) cells play an important role in the innate immune response by directly killing infected and malignant cells and by producing pro- and anti-inflammatory cytokines. Studies on their role in malaria and its complications have resulted in conflicting results.

methodsUsing the commonly used anti-NK1.1 depletion antibodies (PK136) in an in-house optimized experimental model for malaria-associated acute respiratory distress syndrome (MA-ARDS), the role of cNK cells was investigated. Moreover, flow cytometry was performed to characterize different NK cell populations.

resultsWhile cNK cells were found to be dispensable in the development of MA-ARDS, the appearance of a NK1.1

conclusioncNK cells are dispensable in the development of experimental MA-ARDS. Moreover, careful flow cytometric analysis, with a critical mindset in relation to potential aspecific binding despite the use of commercially available Fc blocking reagents, is critical to avoid misinterpretation of the results.

Indexed as

MalariaRespiratory Distress SyndromeAnimalsKiller Cells, NaturalMiceMice, Inbred C57BLMyeloid CellsFlow cytometryLung pathologyMalariaNatural killer cells

Identifiers

PMID38637828
PMCPMC11025177
OpenAlexW4394931435

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.