ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2024
OMIP-102: 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Non-invasive biomarkers to diagnose and monitor eosinophilic esophagitis: a systematic review.Frontiers in medicine · 2025Pooled it
- T cell immunomonitoring: a comparative analysis of traditional and novel methods to quantify and characterize human antigen-specific T cells.Nature protocols · 2026Review
- CellFuse Enables Multimodal Integration of Single-Cell and Spatial Proteomics Data for Systems-Level Analysis in Cancer.Cancer research · 2026Article
- CARGO: A Cytometry Analysis framework via Regularized Graph Optimal-transport.PLoS computational biology · 2026Article
- Chemotherapeutics as Immune Modulators for the Treatment of Triple-Negative Breast Cancer.Cancers · 2026Review
- Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples.Cell reports methods · 2026Article
- Flow Cytometric Screening Reveals Nucleic Acid Dyes Aberrantly Stain Peptidoglycan Sacculi.ACS infectious diseases · 2026Article
- CaptureBody - an anti-CD45 × anti-IgG bispecific antibody enables accurate unmixing for spectral flow cytometry.bioRxiv : the preprint server for biology · 2026Article
- Deconvolution Methods to Link Multi-Omics Data to Cell Type-Specific Extracellular Vesicle Abundances.Proteomics · 2026Review
- Review
- Standardizing a human TBMNK cell assay across instrument platforms: a new framework for producing high-quality, AI-ready reference dataset.Frontiers in immunology · 2026Article
- An optimized three-laser 27-color spectral flow cytometry panel for multi-organ profiling in mice.PloS one · 2026Article
- The changing immune landscape of innate-like T cells and other innate cells throughout life.Immunology and cell biology · 2026Article
- Comprehensive immune profiling of human peripheral blood mononuclear cells using two complementary spectral flow cytometry panels encompassing 60 unique markers.Frontiers in immunology · 2026Article
- Capturing the full spectrum of T cell responses with spectral flow cytometry.Oxford open immunology · 2026Review
- Development and computational analysis of high dimensional spectral flow cytometry data for the resolution of innate lymphoid cells in the mammary tumor microenvironment.Frontiers in immunology · 2026Article
- Key Considerations on CITE-Seq for Single-Cell Multiomics.Proteomics · 2025Review
- Optimization of the Blocking and Signal Preservation Protocol in High-Parameter Flow Cytometry.Current protocols · 2025Article
- Beyond the Limits: How Is Spectral Flow Cytometry Reshaping the Clinical Landscape and What Is Coming Next?Cells · 2025Review
- Photonic microspheres for high-capacity DNA data storage: Robust, straightforward, and scalable random access via nonfading indexes.Science advances · 2025Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
We report the development of an optimized 50-color spectral flow cytometry panel designed for the in-depth analysis of the immune system in human blood and tissues, with the goal of maximizing the amount of information that can be collected using currently available flow cytometry platforms. We established and tested this panel using peripheral blood mononuclear cells (PBMCs), but included CD45 to enable its future use for the analysis of human tissue samples. The panel contains lineage markers for all major immune cell subsets, and an extensive set of phenotyping markers focused on the activation and differentiation status of the T cell and dendritic cell (DC) compartment. We outline the biological insight that can be gained from the simultaneous measurement of such a large number of proteins and propose that this approach provides a unique opportunity for the comprehensive exploration of the immune status in human samples with a limited number of cells. Of note, we tested the panel to be compatible with cell sorting for further downstream applications. Furthermore, to facilitate the wide-spread implementation of such a panel across different cohorts and samples, we established a trimmed-down 45-color version which can be used with different spectral cytometry platforms. Finally, to generate this panel, we utilized not only existing panel design guidelines, but also developed new metrics to systematically identify the optimal combination of 50 fluorochromes and evaluate fluorochrome-specific resolution in the context of a 50-color unmixing matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.