Evidence map›Paper›PMID 38634284›Full record

ArticleAnimal biotechnology2024

Transcriptomic analysis reveals the inhibitory effect of beta-sitosterol on proliferation of bovine preadipocytes.

Lei Jiang, Yuan Wan, Jinhai Pan, Xiaoyu Mao, Xiaolei Sun, Linsen Zan, Hongbao Wang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. The Potential ofInternational journal of molecular sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Lei JiangCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Yuan WanCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Jinhai PanCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Xiaoyu MaoCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Xiaolei SunCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Linsen ZanCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Hongbao WangCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Northwest A&F University · CNNational Beef Cattle Industrial Technology System · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fat deposition affects beef quantity and quality via preadipocyte proliferation. Beta-sitosterol, a natural small molecular compound, has various functions, such as anti-inflammation, antibacterial, and anticancer properties. The mechanism of action of Beta-sitosterol on bovine preadipocytes remains unclear. This study, based on RNA-seq, reveals the impact of Beta -sitosterol on the proliferation of bovine preadipocytes. Compared to the control group, Beta-sitosterol demonstrated a more pronounced inhibitory effect on cell proliferation after 48 hours of treatment than after 24 hours, as evidenced by the results of EdU staining and flow cytometry. RNA-seq and Western Blot analyses further substantiated these findings. Our results suggest that the impact of Beta-sitosterol on the proliferation of bovine preadipocytes is not significant after a 24-hour treatment. It is only after extending the treatment time to 48 hours that Beta-sitosterol may induce cell cycle arrest at the G2/M phase by suppressing the expression of CCNB1, thereby inhibiting the proliferation of bovine preadipocytes.

Indexed as

AdipocytesCell ProliferationSitosterolsAnimalsCattleCells, CulturedGene Expression ProfilingTranscriptomegamma-sitosterolSitosterolsBeta-sitosterolbovine preadipocyteCCNB1cell cycleRNA-seq

Identifiers

PMID38634284
PMCPMC12674350
OpenAlexW4394920997

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.