ArticleBMC plant biology2024
Comparative analysis of chloroplast genomes of Pulsatilla species reveals evolutionary and taxonomic status of newly discovered endangered species Pulsatilla saxatilis.
Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.
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7 citing papers in PubMed.
- Integrating morphology, nuclear SSR, and chloroplast genomes to investigate hybrid origin and species delimitation in the Lycium barbarum L. and its varieties in China.BMC ecology and evolution · 2026Article
- Characterization of the complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2026Article
- Chloroplast genome architecture and phylogenomics of the desert sand-fixing shrubMitochondrial DNA. Part B, Resources · 2026Article
- Hybridization of Lycium qingshuiheense and L. ningxiaense revealed using morphological, SSR markers, and chloroplast genomic data.BMC genomics · 2025Article
- Assembly and comparative analysis of four complete mitochondrial genomes of Pulsatilla species.BMC plant biology · 2025Article
- Characterization and phylogenetic consideration of the complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2025Article
- Correction: Comparative analysis of chloroplast genomes of Pulsatilla species reveals evolutionary and taxonomic status of newly discovered endangered species Pulsatilla saxatilis.BMC plant biology · 2024Article
Corrections and comments
- Erratum issued
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Abstract
backgroundPulsatilla saxatilis, a new species of the genus Pulsatilla has been discovered. The morphological information of this species has been well described, but its chloroplast genome characteristics and comparison with species of the same genus remain to be reported.
resultsOur results showed that the total length of chloroplast (cp.) genome of P. saxatilis is 162,659 bp, with a GC content of 37.5%. The cp. genome contains 134 genes, including 90 known protein-coding genes, 36 tRNA genes, and 8 rRNA genes. P. saxatilis demonstrated similar characteristics to other species of genus Pulsatilla. Herein, we compared cp. genomes of 10 species, including P. saxatilis, and found that the cp. genomes of the genus Pulsatilla are extremely similar, with a length of 162,322-163,851 bp. Furthermore, The SSRs of Pulsatilla ranged from 10 to 22 bp in length. Among the four structural regions of the cp. genome, most long repeats and SSRs were detected in the LSC region, followed by that in the SSC region, and least in IRA/ IRB regions. The most common types of long repeats were forward and palindromic repeats, followed by reverse repeats, and only a few complementary repeats were found in 10 cp. genomes. We also analyzed nucleotide diversity and identified ccsA_ndhD, rps16_trnK-UUU, ccsA, and rbcL, which could be used as potential molecular markers for identification of Pulsatilla species. The results of the phylogenetic tree constructed by connecting the sequences of high variation regions were consistent with those of the cp. gene phylogenetic tree, and the species more closely related to P. saxatilis was identified as the P. campanella.
conclusionIt was determined that the closest species to P. saxatilis is P. campanella, which is the same as the conclusion based on pollen grain characteristics, but different from the P. chinensis determined based on morphological characteristics. By revealing information on the chloroplast characteristics, development, and evolution of the cp. genome and the potential molecular markers, this study provides effective molecular data regarding the evolution, genetic diversity, and species identification of the genus Pulsatilla.
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