Evidence map›Paper›PMID 38630228›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Primary Culture of Dissociated Neurons from the Embryonic Cerebral Cortex.

Shiho Ito, Megumi Kawasaki, Takeshi Kawauchi

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Shiho ItoDepartment of Adaptive and Maladaptive Responses in Health and Disease, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Megumi KawasakiDepartment of Adaptive and Maladaptive Responses in Health and Disease, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Takeshi KawauchiDepartment of Adaptive and Maladaptive Responses in Health and Disease, Graduate School of Medicine, Kyoto University, Kyoto, Japan. kawauchi.takeshi.i92@kyoto-u.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Primary neuronal culture is a valuable in vitro model for analyzing the molecular mechanisms underlying the development and function of neural circuits. In contrast to neurons in vivo, primary cultured neurons can easily be transfected with genes of interest or treated with chemicals such as agonists and inhibitors of a specific target molecule. Furthermore, time-dependent morphological changes, such as the acquisition of neuronal polarity, axon elongation, and dendrite branch formation, can be analyzed by using primary neuronal cultures. Here, we describe a method for preparing a primary culture of neurons from the developing cerebral cortex, together with a method for gene transfer to primary cultured cortical neurons.

Indexed as

AxonsNeuronsCerebral CortexCerebral cortexDissociated cell cultureEmbryonic brainMouseNeuronPrimary culture

Identifiers

PMID38630228

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.