Evidence map›Paper›PMID 38630227›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Generating Homogeneous Brain Organoids from Human iPSCs.

Xianwei Chen, Yanhong Shi

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Xianwei ChenDepartment of Neurodegenerative Diseases, Beckman Research Institute of City of Hope, Duarte, CA, USA.
Yanhong ShiDepartment of Neurodegenerative Diseases, Beckman Research Institute of City of Hope, Duarte, CA, USA. yshi@coh.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is a high demand for the development of in vitro models for human brain development and diseases due to the inaccessibility of human brain tissues. The human iPSC-derived brain organoids provide a promising in vitro model for studying human brain development and disorders. However, it is challenging to generate a large number of brain organoids with high consistency for modeling human neurological diseases. Here, we describe a method for generating high-yield brain organoids with high consistency by combining large-scale embryoid body (EB) generation and incorporating a quality control screening step during differentiation. The method described in this chapter provides a robust way to generate brain organoids for studying human brain development and modeling neurological diseases.

Indexed as

Induced Pluripotent Stem CellsBrainEmbryoid BodiesHumansOrganoidsQuality ControlAlzheimer’s diseaseBrain organoidsDifferentiationDisease modelingEmbryoid bodies (EBs)Induced pluripotent stem cells (iPSCs)Neurological diseasesPluripotent stem cells (PSCs)

Identifiers

PMID38630227

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.