ArticleScientific data2024
High-quality chromosome-level genome assembly of Nicotiana benthamiana.
Article in Scientific data, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- SOBIR1 integrates pathogen recognition and hormonal metabolism in Nicotiana tabacum.Plant physiology · 2026Article
- Endogenous constitutive promoters with a broad range of transcriptional activities for plant synthetic biology.Plant cell reports · 2026Article
- Chromosome-level genome assemblies of Nicotiana attenuata (coyote tobacco) and Nicotiana obtusifolia (desert tobacco).Scientific data · 2026Article
- Improved reconstruction of transcripts and coding sequences from RNA-seq data.Nucleic acids research · 2026Article
- Protocol for triggering plant gene silencing by viral delivery of short RNA.STAR protocols · 2025Article
- Chromosome-scale haploid genome assembly of Durio zibethinus KanYao.Scientific data · 2025Article
- Enhanced Production of Rebaudioside D and Rebaudioside M through V155T Substitution in the Glycosyltransferase UGT91D2 fromJournal of agricultural and food chemistry · 2025Article
- An intron-split microRNA mediates cleavage of the mRNA encoded by low phosphate root in Solanaceae.Planta · 2025Article
- Establishing a comprehensive web-based analysis platform for Nicotiana benthamiana genome and transcriptome.The Plant journal : for cell and molecular biology · 2025Article
- Re-calibration of flow cytometry standards for plant genome size estimation.Frontiers in plant science · 2025Article
- The complete genome assembly of Nicotiana benthamiana reveals the genetic and epigenetic landscape of centromeres.Nature plants · 2024Article
- High-quality chromosome-level genome assembly of Nicotiana benthamiana.Scientific data · 2024Article
- Retrospect and prospect ofFrontiers in plant science · 2024Review
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Authors and funding
8 authors.
Funding
Abstract
Nicotiana benthamiana is a fundamental model organism in plant research. Recent advancements in genomic sequencing have revealed significant intraspecific genetic variations. This study addresses the pressing need for a precise genome sequence specific to its geographic origin by presenting a comprehensive genome assembly of the N. benthamiana LAB strain from the Republic of Korea (NbKLAB). We compare this assembly with the widely used NbLAB360 strain, shedding light on essential genomic differences between them. The outcome is a high-quality, chromosome-level genome assembly comprising 19 chromosomes, spanning 2,762 Mb, with an N50 of 142.6 Mb. Comparative analyses revealed notable variations, including 46,215 protein-coding genes, with an impressive 99.5% BUSCO completeness score. Furthermore, the NbKLAB assembly substantially improved the QV from 33% for NbLAB360 to 49%. This refined chromosomal genome assembly for N. benthamiana, in conjunction with comparative insights, provides a valuable resource for genomics research and molecular biology. This accomplishment forms a strong foundation for in-depth exploration into the intricacies of plant genetics and genomics, improved precision, and a comparative framework.
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