Evidence map›Paper›PMID 38622474›Full record

ReviewMolecular biology reports2024

KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance.

Bhawna Dipta, Salej Sood, Vikas Mangal, Vinay Bhardwaj, Ajay Kumar Thakur, Vinod Kumar, Brajesh Singh

Abstract readReview
PubMed Publisher
In one paragraph

Review in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

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  7. The Bra1K array: an efficient and versatile 1K liquid-phase SNP chip for genetics and breeding in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
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  16. Gene Mapping and Genetic Analysis of Maize Resistance to Stalk Rot.International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Bhawna DiptaICAR-Central Potato Research Institute, Bemloe, Shimla, Himachal Pradesh, 171001, India.ORCID http://orcid.org/0000-0001-7419-7414
Salej SoodICAR-Central Potato Research Institute, Bemloe, Shimla, Himachal Pradesh, 171001, India. salej.sood@icar.gov.in.ORCID http://orcid.org/0000-0002-3163-7501
Vikas MangalICAR-Central Potato Research Institute, Bemloe, Shimla, Himachal Pradesh, 171001, India.ORCID http://orcid.org/0000-0001-9418-2340
Vinay BhardwajICAR-National Research Centre on Seed Spices, Tabiji, Ajmer, Rajasthan, 305206, India.ORCID http://orcid.org/0000-0002-3476-1444
Ajay Kumar ThakurICAR-Central Potato Research Institute, Bemloe, Shimla, Himachal Pradesh, 171001, India.
Vinod KumarICAR-Central Potato Research Institute, Bemloe, Shimla, Himachal Pradesh, 171001, India.
Brajesh SinghICAR-Central Potato Research Institute, Bemloe, Shimla, Himachal Pradesh, 171001, India.ORCID http://orcid.org/0000-0002-6653-0317

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advances in plant molecular breeding have resulted in the development of new varieties with superior traits, thus improving the crop germplasm. Breeders can screen a large number of accessions without rigorous and time-consuming phenotyping by marker-assisted selection (MAS). Molecular markers are one of the most imperative tools in plant breeding programmes for MAS to develop new cultivars possessing multiple superior traits. Single nucleotide polymorphisms (SNPs) are ideal for MAS due to their low cost, low genotyping error rates, and reproducibility. Kompetitive Allele Specific PCR (KASP) is a globally recognized technology for SNP genotyping. KASP is an allele-specific oligo extension-based PCR assay that uses fluorescence resonance energy transfer (FRET) to detect genetic variations such as SNPs and insertions/deletions (InDels) at a specific locus. Additionally, KASP allows greater flexibility in assay design, which leads to a higher success rate and the capability to genotype a large population. Its versatility and ease of use make it a valuable tool in various fields, including genetics, agriculture, and medical research. KASP has been extensively used in various plant-breeding applications, such as the identification of germplasm resources, quality control (QC) analysis, allele mining, linkage mapping, quantitative trait locus (QTL) mapping, genetic map construction, trait-specific marker development, and MAS. This review provides an overview of the KASP assay and emphasizes its validation in crop improvement related to various biotic and abiotic stress tolerance and quality traits.

Indexed as

Plant BreedingPlantsAllelesGenotypePhenotypePolymorphism, Single NucleotideReproducibility of ResultsDisease resistanceDrought toleranceFluorophoreGenotypingKompetitive Allele specific PCR (KASP)

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.