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ArticleParasitology research2024

Molecular detection and genotyping of Dientamoeba fragilis and Blastocystis sp. in housefly Musca domestica (Diptera: Muscidae): first report for Dientamoeba fragilis.

Nuri Ercan, Alparslan Yildirim, Onder Duzlu

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Article in Parasitology research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

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0cells of the map it votes in
4citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Molecular epidemiology and genotype diversity of Dientamoeba fragilis in a Czech hospital-based cohort: higher prevalence in paediatric patients and evidence of mixed G1/G2 infection in a single patient.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Article
  2. Article
  3. Article
  4. Molecular detection ofFood and waterborne parasitology · 2025
    Article
4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Nuri ErcanFaculty of Agriculture, Kirsehir Ahi Evran University, Kirsehir, Turkey. nuri.ercan@ahievran.edu.tr.ORCID http://orcid.org/0000-0001-6039-3510
Alparslan YildirimDepartment of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey.
Onder DuzluDepartment of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey.

Funding

Ahi Evran Üniversitesi KMY.A4.23.002
6 · The paper itself

Abstract

Dientamoeba fragilis and Blastocystis sp. are single-celled protozoan parasites of humans and animals. Although they are found in the intestines of healthy hosts, the pathogenicity of them is still unclear. To date, there is no report on D. fragilis and only two studies (without subtyping) on the occurrence of Blastocystis sp. in Musca domestica. In this study, fly samples were collected from livestock farms and their surroundings in the Kirsehir province (Central Anatolia Region) of Türkiye from May to August 2023. A total of 150 microscopically identified M. domestica samples were analyzed for the detection of D. fragilis and Blastocystis sp. molecularly. The overall prevalence of Blastocystis sp. and D. fragilis in M. domestica was determined to be 3.3% (5/150) and 8.0% (12/150), respectively. The SSU rRNA gene sequences of the isolates indicated genotype 1 of D. fragilis. Eleven isolates were identical and represented a single isolate (KAU-Dfrag1). BLAST analysis of KAU-Dfrag1 indicated identity with the isolates reported from humans, cattle, sheep, and budgerigars. The other isolate (KAU-Dfrag2) was polymorphic at two nucleotides from KAU-Dfrag1 and three nucleotides from known genotypes from GenBank and represented a variant of genotype 1. The Blastocystis sp. isolates were found to be identical and represent a single genotype (KAU-Blast1). BLAST analysis revealed that the KAU-Blast1 genotype belonged to the potentially zoonotic subtype 5 (ST5) and exhibited the highest genetic identity (ranging from 99.4 to 99.6%) with pigs, cattle, and sheep from different countries. Our study provides the first data on the molecular prevalence, epidemiology, and genotypic characterization of D. fragilis and Blastocystis sp. in M. domestica.

Indexed as

BlastocystisBlastocystis InfectionsHousefliesMuscidaeAnimalsCattleDientamoebaFecesGenotypeHumansNucleotidesPrevalenceSheepSwineNucleotidesBlastocystis sp.Dientamoeba fragilisGenotypic characterizationMolecular prevalenceMusca domestica

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.