Evidence map›Paper›PMID 38622287›Full record

ArticleCommunications biology2024

Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology.

Salvatore La Cavera, Veeren M Chauhan, William Hardiman, Mengting Yao, Rafael Fuentes-Domínguez, Kerry Setchfield, Sidahmed A Abayzeed, Fernando Pérez-Cota, Richard J Smith, Matt Clark

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.0field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Salvatore La CaveraOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK. salvatore.lacaveraiii@nottingham.ac.uk.ORCID 0000-0003-0210-3102
Veeren M ChauhanAdvanced Materials & Healthcare Technologies, School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK. veeren.chauhan@nottingham.ac.uk.ORCID 0000-0002-2067-4630
William HardimanOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.ORCID 0000-0002-2452-4752
Mengting YaoOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.ORCID 0000-0003-3378-3358
Rafael Fuentes-DomínguezOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
Kerry SetchfieldOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.ORCID 0000-0002-0339-8494
Sidahmed A AbayzeedOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.ORCID 0000-0002-1379-4001
Fernando Pérez-CotaOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.ORCID 0000-0002-0320-643X
Richard J SmithOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.ORCID 0000-0003-2502-5098
Matt ClarkOptics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.ORCID 0000-0002-1284-3182
University of Nottingham · GB

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5M
NIH HHS P40 OD010440
6 · The paper itself

Abstract

This report presents an optical fibre-based endo-microscopic imaging tool that simultaneously measures the topographic profile and 3D viscoelastic properties of biological specimens through the phenomenon of time-resolved Brillouin scattering. This uses the intrinsic viscoelasticity of the specimen as a contrast mechanism without fluorescent tags or photoacoustic contrast mechanisms. We demonstrate 2 μm lateral resolution and 320 nm axial resolution for the 3D imaging of biological cells and Caenorhabditis elegans larvae. This has enabled the first ever 3D stiffness imaging and characterisation of the C. elegans larva cuticle in-situ. A label-free, subcellular resolution, and endoscopic compatible technique that reveals structural biologically-relevant material properties of tissue could pave the way toward in-vivo elasticity-based diagnostics down to the single cell level.

Indexed as

Imaging, Three-DimensionalMicroscopyAnimalsBiologyCaenorhabditis elegansElasticity

Identifiers

PMID38622287
PMCPMC11018753
OpenAlexW4394813090

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.