ArticleNature communications2024
An experimental framework to assess biomolecular condensates in bacteria.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- Unlocking the bacterial condensome: from "dark matter" to design.Molecular biology of the cell · 2026Article
- Rethinking bioinformatics in liquid-liquid phase separation: data resources, predictive models, and an event-centric perspective.Briefings in bioinformatics · 2026Review
- Optically driven control of mechanochemistry and fusion dynamics of biomolecular condensates via thymine dimerization.Nature communications · 2026Article
- Periplasmic crowding and peptidoglycan hydrolase activity as drivers of outer membrane vesiculation in Acinetobacter baumannii.Communications biology · 2026Article
- Structural and functional implications of phase separation of membrane protein LacY in Escherichia coli.Nature communications · 2026Article
- Spatiotemporal organization of membrane protein controls bacterial extracellular electron transfer.Nature communications · 2026Article
- Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Phase separation and biomolecular condensate formation drive plant endomembrane and autophagy crosstalk.Journal of experimental botany · 2025Review
- Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli.The EMBO journal · 2025Article
- Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.The EMBO journal · 2025Article
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- Cationic Amino Acid Identity and Net Charge Influence Condensate Properties inBiomacromolecules · 2025Article
- Article
- Engineering Spatial Control of Bacterial Organelles.bioRxiv : the preprint server for biology · 2025Article
- Biomolecular Condensates as Emerging Biomaterials: Functional Mechanisms and Advances in Computational and Experimental Approaches.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Current practices in the study of biomolecular condensates: a community comment.Nature communications · 2025Article
- A blueprint for biomolecular condensation driven by bacterial microcompartment encapsulation peptides.Nature communications · 2025Article
- Microscopy-based techniques for studying the material properties of biomolecular condensates in the cellular environment.Biophysical reviews · 2025Review
- Glycogen phase separation drives macromolecular rearrangement and asymmetric division inbioRxiv : the preprint server for biology · 2025Article
- Robust Synthetic Biology Toolkit to Advance Carboxysome Study and Redesign.ACS synthetic biology · 2025Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
High-resolution imaging of biomolecular condensates in living cells is essential for correlating their properties to those observed through in vitro assays. However, such experiments are limited in bacteria due to resolution limitations. Here we present an experimental framework that probes the formation, reversibility, and dynamics of condensate-forming proteins in Escherichia coli as a means to determine the nature of biomolecular condensates in bacteria. We demonstrate that condensates form after passing a threshold concentration, maintain a soluble fraction, dissolve upon shifts in temperature and concentration, and exhibit dynamics consistent with internal rearrangement and exchange between condensed and soluble fractions. We also discover that an established marker for insoluble protein aggregates, IbpA, has different colocalization patterns with bacterial condensates and aggregates, demonstrating its potential applicability as a reporter to differentiate the two in vivo. Overall, this framework provides a generalizable, accessible, and rigorous set of experiments to probe the nature of biomolecular condensates on the sub-micron scale in bacterial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.