Evidence map›Paper›PMID 38621205›Full record

ArticleZebrafish2024

Tagging the

Connor Davison, Hamelynn Harzman, Jessie Nicholson, Seth Entriken, Kierinn Mobley, Abigail Krull, Manik Singhal, Caleb Skow, Nathan Matthews, Lindsey Kopp and 8 more

Open access · greenAbstract read
In one paragraph

Article in Zebrafish, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 1 institution in 1 country.

Connor DavisonDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Hamelynn HarzmanDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Jessie NicholsonDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Seth EntrikenDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Kierinn MobleyDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Abigail KrullDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Manik SinghalDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Caleb SkowDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Nathan MatthewsDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Lindsey KoppDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Benjamin GilletteDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Tyler J WeideDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Jana R HukvariDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Sofia C P StumpfDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Olivia M FeldmannDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Maura McGrailDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.ORCID 0000-0001-9308-6189
Renu SrivastavaDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Jeffrey J EssnerDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.ORCID 0000-0001-8816-3848
Iowa State University · US

Funding

In vivo model of microglia inflammasome signaling in neuroinflammationR24OD020166 · OD · IOWA STATE UNIVERSITY · PI CLARK, KARL J, EKKER, STEPHEN CARL · 2016 to 2023
$7.1M
Conditional genetics and precision genome editing resources for ZebrafishR24OD036201 · OD · IOWA STATE UNIVERSITY · PI KARL J CLARK, Stephen Carl Ekker · 2024 to 2026
$3.0M
NIH HHS R24 OD020166NIH HHS R24 OD036201
6 · The paper itself

Abstract

Tjp1a and other tight junction and adherens proteins play important roles in cell-cell adhesion, scaffolding, and forming seals between cells in epithelial and endothelial tissues. In this study, we labeled Tjp1a of zebrafish with the monomeric red fluorescent protein (mRFP) using CRISPR/Cas9-mediated targeted integration of biotin-labeled polymerase chain reaction (PCR) generated templates. Labeling Tjp1a with RFP allowed us to follow membrane and junctional dynamics of epithelial and endothelial cells throughout zebrafish embryo development. For targeted integration, we used short 35 bp homology arms on each side of the Cas9 genomic target site at the C-terminal of the coding sequence in

Indexed as

CRISPR-Cas SystemsZebrafishAnimalsBiotinEndothelial CellsRed Fluorescent ProteinRNA, Guide, CRISPR-Cas SystemsBiotinRed Fluorescent ProteinRNA, Guide, CRISPR-Cas Systemsbiotin homology armsCRISPR/Cas9targeted integrationzebrafish

Identifiers

PMID38621205
PMCPMC11035848
OpenAlexW4394853594

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.