ArticleMethods in microbiology2015
Low-Density TaqMan® Array Cards for the Detection of Pathogens.
Article in Methods in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Use of TaqMan array card to investigate bacterial isolates in diabetic foot ulcers.Journal of medical microbiology · 2026Article
- Utility of TaqMan Array Cards for detection of acute febrile illness etiologies in patients suspected of viral hemorrhagic fever infections.BMC infectious diseases · 2025Article
- Expanding the wildlife reservoir landscape: A high-throughput molecular surveillance study of zoonotic pathogens in Korean water deer.One health (Amsterdam, Netherlands) · 2025Article
- Customised 96-ocular TaqMan (iCAM) microarray PCR card for rapid diagnosis of microbial keratitis.BMJ open ophthalmology · 2024Article
- Differences in epidemiology of enteropathogens in children pre- and post-rotavirus vaccine introduction in Kilifi, coastal Kenya.Gut pathogens · 2022Article
- Article
- Monitoring of diverse enteric pathogens across environmental and host reservoirs with TaqMan array cards and standard qPCR: a methodological comparison study.The Lancet. Planetary health · 2021Article
- Development and implementation of a customised rapid syndromic diagnostic test for severe pneumonia.Wellcome open research · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time PCR assays have revolutionised diagnostic microbiology over the past 15 years or more. Adaptations and improvements over that time frame have led to the development of multiplex assays. However, limitations in terms of available fluorophores has meant the number of assays which can be combined has remained in single figures. This latter limitation has led to the focus tending to be on individual pathogens and their detection. This chapter describes the development of TaqMan® Array Cards (TACs), technology which allows the detection of multiple pathogens (up to 48 targets) from a single nucleic acid extract, utilising small volumes and real-time PCR. This in turn lends itself to a syndromic approach to infectious disease diagnosis. Using the examples of TACs we have developed in our own laboratory, as well as others, we explain the design, optimisation and use of TACs for respiratory, gastrointestinal and liver infections. Refinement of individual assays is discussed as well as the incorporation of appropriate internal and process controls onto the array cards. Finally, specific examples are given of instances where the assays have had a direct, positive impact on patient care.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.