Evidence map›Paper›PMID 38617372›Full record

ArticlebioRxiv : the preprint server for biology2024

3D Imaging Reveals Changes in the Neurovascular Architecture of the Murine Calvarium with Aging.

Allison L Horenberg, Yunke Ren, Alexandra N Rindone, Arvind P Pathak, Warren L Grayson

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Allison L HorenbergDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Yunke RenDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Alexandra N RindoneDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Arvind P PathakDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Warren L GraysonDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID 0000-0001-6099-6469

Funding

Multiscale Image-based Modeling of Antiangiogenic Resistance in Breast CancerR01CA196701 · NCI · JOHNS HOPKINS UNIVERSITY · PI Arvind P Pathak · 2015 to 2026
$3.6M
Next-Gen Oncosurveillance: Wireless Multi-Function Microscopy of In Vivo Brain Cancer DynamicsR01CA237597 · NCI · JOHNS HOPKINS UNIVERSITY · PI Arvind P Pathak · 2020 to 2026
$2.6M
Oxygen-eluting scaffolds for cranial bone regenerationR01DE027957 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI GRAYSON, WARREN L, PATHAK, ARVIND P · 2019 to 2023
$2.4M
NCI NIH HHS R01 CA196701NCI NIH HHS R01 CA237597NIDCR NIH HHS R01 DE027957
6 · The paper itself

Abstract

Calvarial nerves, along with vasculature, influence skull formation during development and following injury, but it remains unclear how calvarial nerves are spatially distributed during postnatal growth and aging. Studying the spatial distribution of nerves in the skull remains challenging due to a lack of methods to image and quantify 3D structures in intact bone. To visualize calvarial 3D neurovascular architecture, we imaged nerves and endothelial cells with lightsheet microscopy. We employed machine-learning-based segmentation to facilitate high-resolution characterization from post-natal day 0 (P0) to Week 80 (80wk). We found that TUBB3+ nerve density decreased with aging with the frontal bone demonstrating earlier onset age-related nerve loss than the parietal bone. In addition, nerves in the periosteum and dura mater exhibited similar yet distinct temporal patterns of nerve growth and loss. While no difference was observed in TUBB3+ nerves during skeletal maturation (P0 → 12wk), we did observe an increase in the volume of unmyelinated nerves in the dura mater. Regarding calvarial vasculature, larger CD31

Indexed as

AgingBoneLightsheet MicroscopyNervesNeurovascular

Identifiers

PMID38617372
PMCPMC11014476

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.