Evidence map›Paper›PMID 38617369›Full record

ArticlebioRxiv : the preprint server for biology2024

Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting.

R Jaiswal, V Santosh, B Braud, A Washington, Carlos R Escalante

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

R JaiswalDepartment of Physiology and Biophysics, Virginia Commonwealth University, School of Medicine, Richmond VA, 23298.
V SantoshDepartment of Physiology and Biophysics, Virginia Commonwealth University, School of Medicine, Richmond VA, 23298.
B BraudDepartment of Physiology and Biophysics, Virginia Commonwealth University, School of Medicine, Richmond VA, 23298.
A WashingtonDepartment of Physiology and Biophysics, Virginia Commonwealth University, School of Medicine, Richmond VA, 23298.
Carlos R EscalanteDepartment of Physiology and Biophysics, Virginia Commonwealth University, School of Medicine, Richmond VA, 23298.ORCID 0000-0001-5648-3361
Virginia Commonwealth University · USMayo Clinic · USUnited States Department of the Army · US

Funding

Pacific Northwest Center for Cryo-EM - Screening supplementU24GM129547 · NIGMS · OREGON HEALTH & SCIENCE UNIVERSITY · PI EVANS, JAMES E, GOUAUX, JAMES E · 2018 to 2023
$54.8M
Structural and Mechanistic Insights into AAV Rep Mediated Site-Specific Integration and PackagingR01GM124204 · NIGMS · VIRGINIA COMMONWEALTH UNIVERSITY · PI Carlos R Escalante · 2017 to 2026
$4.2M
NIGMS NIH HHS R01 GM124204NIGMS NIH HHS U24 GM129547
6 · The paper itself

Abstract

The Rep68 protein from Adeno-Associated Virus (AAV) is a multifunctional SF3 helicase that performs most of the DNA transactions required for the viral life cycle. During AAV DNA replication, Rep68 assembles at the origin and catalyzes the DNA melting and nicking reactions during the hairpin rolling replication process to complete the second-strand synthesis of the AAV genome. Here, we report the Cryo-EM structures of Rep68 bound to double-stranded DNA (dsDNA) containing the sequence of the AAVS1 integration site in different nucleotide-bound states. In the apo state, Rep68 forms a heptameric complex around DNA, with three Origin Binding Domains (OBDs) bound to the Rep Binding Site (RBS) sequence and three other OBDs forming transient dimers with them. The AAA

Indexed as

AAV Integration site 1Adeno-Associated VirusElectron MicroscopyProtein-DNA interaction

Identifiers

PMID38617369
PMCPMC11014581
OpenAlexW4393868266

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.