ArticlebioRxiv : the preprint server for biology2024
A specific and portable gene expression program underlies antigen archiving by lymphatic endothelial cells.
Ryan M Sheridan, Thu A Doan, Cormac Lucas, Tadg S Forward, Aspen Uecker-Martin, Thomas E Morrison, Jay R Hesselberth, Beth A Jirón Tamburini
Open access · greenAbstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 · The registryThe trial behind it
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4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
8 authors at 3 institutions in 1 country.
Ryan M SheridanDepartment of Biochemistry and Molecular Genetics, RNA Bioscience Initiative, University of Colorado School of Medicine.
Thu A DoanDepartment of Medicine, Division of Gastroenterology and Hepatology, University of Colorado School of Medicine.
Cormac LucasDepartment of Immunology and Microbiology, Aurora, CO, USA.
Tadg S ForwardDepartment of Medicine, Division of Gastroenterology and Hepatology, University of Colorado School of Medicine.
Aspen Uecker-MartinDepartment of Biochemistry and Molecular Genetics, RNA Bioscience Initiative, University of Colorado School of Medicine.
Jay R HesselberthDepartment of Biochemistry and Molecular Genetics, RNA Bioscience Initiative, University of Colorado School of Medicine.ORCID 0000-0002-6299-179X Beth A Jirón TamburiniDepartment of Medicine, Division of Gastroenterology and Hepatology, University of Colorado School of Medicine.ORCID 0000-0003-1991-231X University of Colorado Anschutz Medical Campus · USThe Medical Center of Aurora · USUniversity of Colorado Denver · US
Funding
Nanopore analysis of transfer RNA forms and functionsR35GM119550 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Jay R Hesselberth · 2016 to 2026
$4.5MCooperation between lymphatic stroma and hematopoietic cells shapes protective immunityR01AI121209 · NIAID · UNIVERSITY OF COLORADO DENVER · PI Beth Ann Tamburini · 2016 to 2026
$4.5MPredoctoral Training Program in Molecular and Cellular Biology (Supplement: Mentoring in the Research Environment)T32GM136444 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI MICHAEL A MCMURRAY, Rytis Prekeris · 2020 to 2026
$3.7MBiochemistry at single-cell resolution: a new approach to understand functional heterogeneityR01AG071467 · NIA · UNIVERSITY OF COLORADO DENVER · PI HESSELBERTH, JAY R · 2020 to 2024
$3.2MPD-L1 reverse signaling in dermal DCs promotes DC migration and skin immunity to cutaneous pathogensR01AI155474 · NIAID · UNIVERSITY OF COLORADO DENVER · PI TAMBURINI, BETH ANN · 2020 to 2024
$2.3MMolecular tracking of antigen following vaccinationR21AI155929 · NIAID · UNIVERSITY OF COLORADO DENVER · PI TAMBURINI, BETH ANN · 2021 to 2022
$417kNIAID NIH HHS R01 AI121209NIAID NIH HHS R01 AI155474NIAID NIH HHS R21 AI155929NIA NIH HHS R01 AG071467NIGMS NIH HHS R35 GM119550NIGMS NIH HHS T32 GM136444
6 · The paper itselfAbstract
Antigens from protein subunit vaccination traffic from the tissue to the draining lymph node, either passively via the lymph or carried by dendritic cells at the local injection site. Lymph node (LN) lymphatic endothelial cells (LEC) actively acquire and archive foreign antigens, and archived antigen can be released during subsequent inflammatory stimulus to improve immune responses. Here, we answer questions about how LECs achieve durable antigen archiving and whether there are transcriptional signatures associated with LECs containing high levels of antigen. We used single cell sequencing in dissociated LN tissue to quantify antigen levels in LEC and dendritic cell populations at multiple timepoints after immunization, and used machine learning to define a unique transcriptional program within archiving LECs that can predict LEC archiving capacity in independent data sets. Finally, we validated this modeling, showing we could predict antigen archiving from a transcriptional dataset of CHIKV infected mice and demonstrated
Indexed as
antigen archivingChikungunya virusdendritic cellfibroblastic reticular cellgene expression programimmunizationlymphatic endothelial celllymph nodelymph node stromal cell
Identifiers
PMID38617225
PMCPMC11014631
OpenAlexW4393578651
What OpenQuestion holds
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LicenceCC BY-NC-ND
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