ArticleTicks and tick-borne diseases2024
A next generation sequencing assay combining Ixodes species identification with pathogen detection to support tick surveillance efforts in the United States.
Article in Ticks and tick-borne diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Efficacy of a low-dose fipronil bait in controlling blacklegged tick (Ixodes scapularis) larvae parasitizing white-footed mice (Peromyscus leucopus) under field conditions in the northeastern United States.Parasites & vectors · 2026Article
- High throughput sequencing in the diagnosis and surveillance of tick-borne diseases: A narrative review.One health (Amsterdam, Netherlands) · 2026Review
- Article
- Acquisition of Borrelia burgdorferi sensu stricto (Spirochaetales: Spirochaetaceae) by Haemaphysalis longicornis (Acari: Ixodidae) nymphs during interrupted feeding.Journal of medical entomology · 2025Article
- Ticks and tick-borne diseases in the northern hemisphere affecting humans.Frontiers in microbiology · 2025Review
- Geographic variation in the distribution of Anaplasma phagocytophilum variants in host-seeking Ixodes scapularis nymphs and adults in the eastern United States elucidated using next generation sequencing.Ticks and tick-borne diseases · 2024Article
- Update on tick-borne pathogens detection methods within ticks.Current research in parasitology & vector-borne diseases · 2024Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The burden of tick-borne diseases continues to increase in the United States. Tick surveillance has been implemented to monitor changes in the distribution and prevalence of human disease-causing pathogens in ticks that frequently bite humans. Such efforts require accurate identification of ticks to species and highly sensitive and specific assays that can detect and differentiate pathogens from genetically similar microbes in ticks that have not been demonstrated to be pathogenic in humans. We describe a modification to a next generation sequencing pathogen detection assay that includes a target that accurately identifies Ixodes ticks to species. We show that the replacement of internal control primers used to ensure assay performance with primers that also act as an internal control and can additionally differentiate tick species, retains high sensitivity and specificity, improves efficiency, and reduces costs by eliminating the need to run separate assays to screen for pathogens and for tick identification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.