Evidence map›Paper›PMID 38615284›Full record

ArticleThe protein journal2024

Glucose-Binding Dioclea bicolor Lectin (DBL): Purification, Characterization, Structural Analysis, and Antibacterial Properties.

Willian F Reis, Marcos E S Silva, Ana C S Gondim, Renato C F Torres, Rômulo F Carneiro, Celso S Nagano, Alexandre H Sampaio, Claudener S Teixeira, Lenita C B F Gomes, Bruno L Sousa and 3 more

Abstract read
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In one paragraph

Article in The protein journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Characterization of a NovelInternational journal of molecular sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Willian F ReisDepartamento de Ciências da Natureza E da Terra, Universidade Do Estado de Minas Gerais, Unidade de Divinópolis, Divinópolis, MG, Brazil.
Marcos E S SilvaFaculdade de Educação de Itapipoca, Universidade Estadual Do Ceará, Itapipoca, CE, Brazil.
Ana C S GondimDepartamento de Química Orgânica E Inorgânica, Universidade Federal Do Ceará, Fortaleza, CE, Brazil.
Renato C F TorresCentro de Ciências Agrárias E da Biodiversidade, Universidade Federal Do Cariri, Crato, CE, Brazil.
Rômulo F CarneiroLaboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal Do Ceará, Fortaleza, CE, Brazil.
Celso S NaganoLaboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal Do Ceará, Fortaleza, CE, Brazil.
Alexandre H SampaioLaboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal Do Ceará, Fortaleza, CE, Brazil.
Claudener S TeixeiraCentro de Ciências Agrárias E da Biodiversidade, Universidade Federal Do Cariri, Crato, CE, Brazil.
Lenita C B F GomesFaculdade de Filosofia Dom Aureliano Matos, Universidade Estadual Do Ceará, Limoeiro Do Norte, CE, Brazil.
Bruno L SousaFaculdade de Filosofia Dom Aureliano Matos, Universidade Estadual Do Ceará, Limoeiro Do Norte, CE, Brazil.
Alexandre L AndradeLaboratório Integrado de Biomoléculas - LIBS, Departamento de Patologia E Medicina Legal, Universidade Federal Do Ceará, Fortaleza, CE, Brazil.
Edson H TeixeiraLaboratório Integrado de Biomoléculas - LIBS, Departamento de Patologia E Medicina Legal, Universidade Federal Do Ceará, Fortaleza, CE, Brazil.
Mayron A VasconcelosDepartamento de Ciências da Natureza E da Terra, Universidade Do Estado de Minas Gerais, Unidade de Divinópolis, Divinópolis, MG, Brazil. mayronvasconcelos@gmail.com.
Universidade Federal do Ceará · BRUniversidade Estadual do Ceará · BRUniversidade Federal do Cariri · BRNatura (Brazil) · BRUniversidade do Estado de Minas Gerais · BR

Funding

Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-00224-21
6 · The paper itself

Abstract

In this study, we purified a lectin isolated from the seeds of Dioclea bicolor (DBL) via affinity purification. Electrophoresis analysis revealed that DBL had three bands, α, β, and γ chains, with molecular masses of approximately 29, 14, and 12 kDa, respectively. Gel filtration chromatography revealed that the native form of DBL had a molecular mass of approximately 100 kDa, indicating that it is a tetramer. Interestingly, DBL-induced hemagglutination was inhibited by several glucosides, mannosides, ampicillin, and tetracycline with minimum inhibitory concentration (MIC) values of 1.56-50 mM. Analysis of the complete amino acid sequence of DBL revealed the presence of 237 amino acids with high similarity to other Diocleinae lectins. Circular dichroism showed the prominent β-sheet secondary structure of DBL. Furthermore, DBL structure prediction revealed a Discrete Optimized Protein Energy (DOPE) score of -26,642.69141/Normalized DOPE score of -1.84041. The DBL monomer was found to consist a β-sandwich based on its 3D structure. Molecular docking showed the interactions between DBL and α-D-glucose, N-acetyl-D-glucosamine, α-D-mannose, α-methyl-D-mannoside, ampicillin, and tetracycline. In addition, DBL showed antimicrobial activity with an MIC of 125 μg/mL and exerted synergistic effects in combination with ampicillin and tetracycline (fractional inhibitory concentration index ≤ 0.5). Additionally, DBL significantly inhibited biofilm formation and showed no toxicity in murine fibroblasts (p < 0.05). These results suggest that DBL exhibits antimicrobial activity and works synergistically with antibiotics.

Indexed as

Anti-Bacterial AgentsDiocleaPlant LectinsAmpicillinAnimalsMiceMicrobial Sensitivity TestsMolecular Docking SimulationAmpicillinAnti-Bacterial AgentsPlant LectinsAntimicrobial activityConA-likeDioclea bicolorStructural characterizationSynergistic effect

Identifiers

PMID38615284
OpenAlexW4394794078

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.