Evidence map›Paper›PMID 38613650›Full record

ArticleJournal of assisted reproduction and genetics2024

The utility of human two plus one small pronucleated zygotes (2.1PN) based on clinical outcomes and the focused ploidy analysis.

Hiromitsu Hattori, Noriyuki Okuyama, Kyota Ashikawa, Yoshiyuki Sakuraba, Hideki Igarashi, Koichi Kyono

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hiromitsu HattoriKyono ART Clinic Sendai, 1-1-1 3F, Honcho, Aobaku, Sendai, Miyagi, 980-0014, Japan.
Noriyuki OkuyamaKyono ART Clinic Takanawa, Takanawa Court 5F, 3-13-1, Takanawa Tokyo, Minatoku, 108-0074, Japan.
Kyota AshikawaVarinos Inc, DiverCity Tokyo Office Tower 21F, 1-1-20 Aomi, Koutou-Ku, Tokyo, Japan.
Yoshiyuki SakurabaVarinos Inc, DiverCity Tokyo Office Tower 21F, 1-1-20 Aomi, Koutou-Ku, Tokyo, Japan.
Hideki IgarashiKyono ART Clinic Sendai, 1-1-1 3F, Honcho, Aobaku, Sendai, Miyagi, 980-0014, Japan.
Koichi KyonoKyono ART Clinic Sendai, 1-1-1 3F, Honcho, Aobaku, Sendai, Miyagi, 980-0014, Japan. kyono@ivf-kyono.or.jp.ORCID http://orcid.org/0000-0001-5211-7778

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeAre human embryos arising from two plus one small pronucleated zygotes, called 2.1 pronuclei (PN), clinically useful?

methodsIn a retrospective embryo cohort study and prospective experimental study, a total of 287 cycles in which at least one 2.1PN was identified in the fertilization check were included. Embryonic development and clinical outcome were compared for the 1395 2PN zygotes and 304 2.1PN zygotes that were siblings. All embryos were individually cultured in time-lapse systems. Twenty-five 2.1PN-derived blastocysts, donated for research, were used in focused single-nucleotide variant ploidy analysis to identify the distribution pattern of heterozygosity.

resultsThe average diameter of PN was 24.9 ± 2.4 µm for large PN and 10.2 ± 2.4 µm for small PN; 79.9% of small PN was derived from female pronuclei. Blastocyst formation rate and good-quality blastocyst rate were significantly lower with 2.1PN embryos than with 2PN embryos (40.0% vs. 57.7%, 21.4% vs. 33.5%, respectively). A total of 13 embryos derived from 2.1PN were transferred, and three healthy babies were born. In ploidy constitutions of trophectoderm (TE), 2.1PN-derived blastocyst TE was shown to be mostly diploid (95.8%, 23/24), and only one blastocyst showed triploid.

conclusionsIt was suggested that 2.1PN embryos have lower embryonic developmental potential than 2PN embryos, but most of the 2.1PN were diploid, indicating that they are likely to be clinically usable. It is recommended to perform embryo transfer following a combination of PGT-A and ploidy analysis.

Indexed as

BlastocystEmbryonic DevelopmentEmbryo TransferFertilization in VitroPloidiesPregnancy RateZygoteAdultCell NucleusEmbryo Culture TechniquesFemaleHumansMalePregnancyPreimplantation DiagnosisProspective StudiesEmbryo developmentEmbryo haplotypingLive birthPreimplantation genetic testingTwo plus one small pronucleated zygotes (2.1PN)

Identifiers

PMID38613650
PMCPMC11224203

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.