Stress-Induced Changes in Nucleocytoplasmic Localization of Crucial Factors in Gene Expression Regulation.

Ali Khamit, Payal Chakraborty, Szabolcs Zahorán, Zoltán Villányi, Hajnalka Orvos, Edit Hermesz

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Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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6 authors at 1 institution in 1 country.

Ali KhamitDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, H-6701 Szeged, Hungary.ORCID 0000-0002-5405-7702

Payal ChakrabortyDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, H-6701 Szeged, Hungary.ORCID 0000-0001-5471-2888

Szabolcs ZahoránDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, H-6701 Szeged, Hungary.ORCID 0000-0002-6649-8249

Zoltán VillányiDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, H-6701 Szeged, Hungary.ORCID 0000-0002-8563-9713

Hajnalka OrvosDepartment of Obstetrics and Gynecology, Albert Szent-Györgyi Medical School, University of Szeged, H-6701 Szeged, Hungary.

Edit HermeszDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, H-6701 Szeged, Hungary.ORCID 0000-0002-0543-6693

University of Szeged · HU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigates the toxic effect of harmful materials, unfiltered by the placenta, on neonatal umbilical cord (UC) vessels, focusing on stress-induced adaptations in transcriptional and translational processes. It aims to analyze changes in pathways related to mRNA condensate formation, transcriptional regulation, and DNA damage response under maternal smoking-induced stress. UC vessels from neonates born to smoking (Sm) and nonsmoking mothers (Ctr) were examined. Immunofluorescence staining and confocal microscopy assessed the localization of key markers, including Transcription Complex Subunit 1 (CNOT1) and the largest subunit of RNA polymerase II enzyme (RPB1). Additionally, markers of DNA damage response, such as Poly(ADP-ribose) polymerase-1, were evaluated. In Sm samples, dissolution of CNOT1 granules in UC vessels was observed, potentially aiding stalled translation and enhancing transcription via RPB1 assembly and translocation. Control vessels showed predominant cytoplasmic RPB1 localization. Despite adaptive responses, Sm endothelial cells exhibited significant damage, indicated by markers like Poly(ADP-ribose) polymerase-1. Ex vivo metal treatment on control vessels mirrored Sm sample alterations, emphasizing marker roles in cell survival under toxic exposure. Maternal smoking induces specific molecular adaptations in UC vessels, affecting mRNA condensate formation, transcriptional regulation, and DNA damage response pathways. Understanding these intricate molecular mechanisms could inform interventions to improve neonatal health outcomes and mitigate adverse effects of toxic exposure during pregnancy.

Indexed as

Cone-Rod DystrophiesEndothelial CellsFemaleGene Expression RegulationHumansInfant, NewbornPoly(ADP-ribose) PolymerasesPregnancyRNA, MessengerTranscription FactorsTranscription, GeneticCNOT1 protein, humanPoly(ADP-ribose) PolymerasesRNA, MessengerTranscription Factorsblood vesselsCNOT1 (carbon catabolite repression-negative on TATA-less)fetal developmentmaternal smokingPARP-1 (poly-(ADP-ribose) polymerase-1)rescue mechanismRPB1 (the largest subunit of RNA polymerase II enzyme)

Identifiers

PMID38612704

DOI10.3390/ijms25073895

PMCPMC11012061

OpenAlexW4393356641

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.