Evidence map›Paper›PMID 38606634›Full record

ArticleOtolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery2024

A Comprehensive Flow Cytometry Panel for Analysis of Idiopathic Subglottic Stenosis.

Raymond J So, Samuel L Collins, Yee Chan-Li, Ioan Lina, Alexander Gelbard, Kevin M Motz, Alexander T Hillel

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Raymond J SoDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.ORCID http://orcid.org/0000-0003-1004-7606
Samuel L CollinsDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Yee Chan-LiDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Ioan LinaDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Alexander GelbardDepartment of Otolaryngology-Head and Neck Surgery, Vanderbilt University, Nashville, Tennessee, USA.
Kevin M MotzDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Alexander T HillelDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Johns Hopkins University · USVanderbilt University · US

Funding

Targeting mTOR regulation of T-lymphocytes and fibroblasts in Laryngotracheal StenosisR01DC018567 · NIDCD · JOHNS HOPKINS UNIVERSITY · PI HILLEL, ALEXANDER · 2020 to 2024
$3.8M
Targeting pathologic macrophage activation through inhibition of MyD88 to attenuate laryngotracheal stenosisK23DC020988 · NIDCD · JOHNS HOPKINS UNIVERSITY · PI Kevin Motz · 2023 to 2026
$860k
Role of E-cadherin in epithelial barrier dysfunction and fibrosis in idiopathic subglottic stenosisR56DC020736 · NIDCD · JOHNS HOPKINS UNIVERSITY · PI HILLEL, ALEXANDER · 2023 to 2023
$154k
NIDCD NIH HHS K23 DC020988NIDCD NIH HHS R01 DC018567NIDCD NIH HHS R56 DC020736
6 · The paper itself

Abstract

objectiveTo present a comprehensive flow cytometry panel for idiopathic subglottic stenosis (iSGS). STUDY

designControlled ex vivo cohort study.

settingTertiary care academic hospital in a metropolitan area.

methodsFlow cytometry and single-cell RNA sequencing were performed on 9 paired normal and scar tissue samples from iSGS patients. Flow cytometry was used to assess the presence of myeloid (CD11b, CD14, CD15, Siglec8), lymphoid (CD3, CD4, CD8, gamma delta [γδ], FOXP3), endothelial (CD31), fibroblast (CD90, SMA), and epithelial (CD326, CK5) markers.

resultsOn flow cytometry, iSGS scar is characterized by an increased presence of myeloid, lymphoid, endothelial, and fibroblast cell types, but a decreased presence of epithelial cells. In the myeloid lineage, iSGS scar samples demonstrated increased CD11b

conclusionWe present a comprehensive flow cytometry panel for iSGS. This flow panel may serve as a common platform among airway scientists to elucidate the cellular mechanisms underpinning iSGS and other upper airway pathologies. Scar iSGS samples demonstrate a distinct cellular profile relative to normal iSGS specimens, exhibiting increased fibroblast, endothelial, and inflammatory cell types but decreased epithelium.

Indexed as

Flow CytometryLaryngostenosisBiomarkersCicatrixCohort StudiesFemaleHumansMaleMiddle AgedBiomarkersflow cytometryidiopathic subglottic stenosislaryngotracheal stenosissingle‐cell RNA sequencing

Identifiers

PMID38606634
PMCPMC11349474
OpenAlexW4394762638

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.