ArticleFrontiers in bioengineering and biotechnology2024
A putative design for the electromagnetic activation of split proteins for molecular and cellular manipulation.
Article in Frontiers in bioengineering and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Magneto-Photonic Gene Circuit for Minimally Invasive Control of Gene Expression in Mammalian Cells.ACS omega · 2026Article
- Magneto-Photonic Gene Circuit for Minimally Invasive Control of Gene Expression in Mammalian Cells.bioRxiv : the preprint server for biology · 2025Article
- Genetics-Based Targeting Strategies for Precise Neuromodulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Using Manganese-Enhanced MRI to visualize Magnetogenetic-based Neuromodulation.bioRxiv : the preprint server for biology · 2025Article
- Open-source magnetic system for wireless neuromodulations in vitro and for untethered brain stimulation in vivo.Scientific reports · 2025Article
- Photocontrolling the Enantioselectivity of a Phosphotriesterase via Incorporation of a Light-Responsive Unnatural Amino Acid.JACS Au · 2025Article
- A conserved phenylalanine motif among teleost fish provides insight for improving electromagnetic perception.Open biology · 2024Article
- A conserved phenylalanine motif among Teleost fish provides insight for improving electromagnetic perception.bioRxiv : the preprint server for biology · 2024Article
- Magnetogenetic stimulation inside MRI induces spontaneous and evoked changes in neural circuits activity in rats.Frontiers in neuroscience · 2024Article
- Theranostics - a sure cure for cancer after 100 years?Theranostics · 2024Review
- Magnetogenetic stimulation inside MRI induces spontaneous and evoked changes in neural circuits activity in rats.bioRxiv : the preprint server for biology · 2023Article
- Proposed three-phenylalanine motif involved in magnetoreception signalling of an Actinopterygii protein expressed in mammalian cells.Open biology · 2023Article
- A Genetic Programming Approach to Engineering MRI Reporter Genes.ACS synthetic biology · 2023Article
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Authors and funding
6 authors.
Funding
Abstract
The ability to manipulate cellular function using an external stimulus is a powerful strategy for studying complex biological phenomena. One approach to modulate the function of the cellular environment is split proteins. In this method, a biologically active protein or an enzyme is fragmented so that it reassembles only upon a specific stimulus. Although many tools are available to induce these systems, nature has provided other mechanisms to expand the split protein toolbox. Here, we show a novel method for reconstituting split proteins using magnetic stimulation. We found that the electromagnetic perceptive gene (EPG) changes conformation due to magnetic field stimulation. By fusing split fragments of a certain protein to both termini of the EPG, the fragments can be reassembled into a functional protein under magnetic stimulation due to conformational change. We show this effect with three separate split proteins: NanoLuc, APEX2, and herpes simplex virus type-1 thymidine kinase. Our results show, for the first time, that reconstitution of split proteins can be achieved only with magnetic fields. We anticipate that this study will be a starting point for future magnetically inducible split protein designs for cellular perturbation and manipulation. With this technology, we can help expand the toolbox of the split protein platform and allow better elucidation of complex biological systems.
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