Evidence map›Paper›PMID 38602296›Full record

ArticleNano letters2024

Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM.

Ali Khoshouei, Georg Kempf, Volodymyr Mykhailiuk, Johanna Mariko Griessing, Maximilian Nicolas Honemann, Lukas Kater, Simone Cavadini, Hendrik Dietz

Abstract read
In one paragraph

Article in Nano letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Recent Advances in Aptamer-Based Applications in Cardiology.International journal of molecular sciences · 2026
    Review
  5. Article
  6. Isothermal assembly of DNA nanostructures.Chemical communications (Cambridge, England) · 2025
    Review
  7. Article
  8. Crystalline Assemblies of DNA Nanostructures and Their Functional Properties.Angewandte Chemie (International ed. in English) · 2025
    Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ali KhoshoueiLaboratory for Biomolecular Nanotechnology, Department of Biosciences, School of Natural Sciences, Technical University of Munich, Am Coulombwall 4a, 85748 Garching, Germany.ORCID 0000-0003-1735-8423
Georg KempfFriedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.ORCID 0000-0002-0228-6730
Volodymyr MykhailiukLaboratory for Biomolecular Nanotechnology, Department of Biosciences, School of Natural Sciences, Technical University of Munich, Am Coulombwall 4a, 85748 Garching, Germany.ORCID 0000-0001-9928-3845
Johanna Mariko GriessingLaboratory for Biomolecular Nanotechnology, Department of Biosciences, School of Natural Sciences, Technical University of Munich, Am Coulombwall 4a, 85748 Garching, Germany.ORCID 0009-0003-0828-4749
Maximilian Nicolas HonemannLaboratory for Biomolecular Nanotechnology, Department of Biosciences, School of Natural Sciences, Technical University of Munich, Am Coulombwall 4a, 85748 Garching, Germany.ORCID 0000-0001-7805-906X
Lukas KaterFriedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.
Simone CavadiniFriedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.ORCID 0000-0003-2777-7584
Hendrik DietzLaboratory for Biomolecular Nanotechnology, Department of Biosciences, School of Natural Sciences, Technical University of Munich, Am Coulombwall 4a, 85748 Garching, Germany.ORCID 0000-0003-1270-3662

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular functionalities in a programmable manner. In this study, we examined the impact of internal crossover distribution and the compositional uniformity of staple strands on the structure of multilayer DNA origami using cryogenic electron microscopy (cryo-EM) single-particle analysis. A refined DNA object was utilized as an alignment framework in a host-guest model, where we successfully resolved an 8 kDa thrombin binding aptamer (TBA) linked to the host object. Our results broaden the spectrum of DNA in structural applications.

Indexed as

Cryo-EMDesign OptimizationDNA origamiScaffoldingThrombin Binding Aptamer

Identifiers

PMID38602296
PMCPMC11057029

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.