ArticleVirology journal2024
Immunopathological investigation and genetic evolution of Avian leukosis virus Subgroup-J associated with myelocytomatosis in broiler flocks in Egypt.
Article in Virology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 14 citations in OpenAlex.
- Multiple cases of osteopetrosis-like disease in young broiler flocks in Germany.Poultry science · 2026Article
- First report of molecular genotyping, pathotyping, and histopathological characterization of lentogenic genotype II Newcastle disease virus circulating in young ostrich flocks in Egypt.Veterinary world · 2026Article
- Pathological mechanisms of avian viral oncogenesis: The central role of MicroRNA.Poultry science · 2026Review
- Serologic and molecular evidence of avian metapneumovirus subtypes A and B in unvaccinated broiler breeder flocks in Egypt (2024-2025).BMC veterinary research · 2026Article
- Acetylation-mediated regulation of ALV viral proteins: Implications for retroviral inhibition.PLoS pathogens · 2026Article
- Illuminating the pathological, immunohistochemical, and molecular investigations of myelocytomatosis in chicken embryos.Infectious agents and cancer · 2026Article
- Histopathological diagnosis and genetic diversity of avian encephalomyelitis virus circulating in broiler farms in Egypt.Open veterinary journal · 2026Article
- Seromolecular and histopathological study on Toxoplasma gondii infection in ruminants in Aswan, Egypt.BMC veterinary research · 2025Article
- Genomic and evolutionary characterization of newly emerged highly pathogenic avian influenza H5N1 clade (2023-2025).Veterinary world · 2025Article
- Virological perspectives of the current situation of oncogenic viruses in Egypt: a review.Infectious agents and cancer · 2025Review
- Article
- Molecular characterization and immunopathological investigation of Avian reticuloendotheliosis virus in breeder flocks in Egypt.Virology journal · 2024Article
- Pathological and immunohistochemical studies of lymphoid leukosis in pigeons in Egypt.Open veterinary journal · 2024Article
- Exosomal miR-7-25207 Increases Subgroup J Avian Leukosis Virus Titers by Targeting the Akt-CyclinQ1 and PRC1-YAF2 Dual Pathways.Microorganisms · 2024Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAvian leukosis virus Subgroup-J (ALV-J) is a rapidly oncogenic evolving retrovirus infecting a variety of avian species; causing severe economic losses to the local poultry industry.
methodsTo investigate ALV-J, a total of 117 blood samples and 57 tissue specimens of different organs were collected for virological, and pathological identification, serological examinations, molecular characterization, and sequencing analysis. To the best of our knowledge, this is the first detailed report recorded in broiler flocks in Egypt. The present study targets the prevalence of a viral tumor disease circulating in broiler flocks in the El-Sharqia, El-Dakahliya, and Al-Qalyubiyya Egyptian governorates from 2021 to 2023 using different diagnostic techniques besides ALV-J gp85 genetic diversity determination.
resultWe first isolated ALV-J on chicken embryo rough cell culture; showing aggregation, rounding, and degeneration. Concerning egg inoculation, embryonic death, stunting, and curling were observed. Only 79 serum samples were positive for ALV-J (67.52%) based on the ELISA test. Histopathological investigation showed tumors consist of uniform masses, usually well-differentiated myelocytes, lymphoid cells, or both in the liver, spleen, and kidneys. Immunohistochemical examination showed that the myelocytomatosis-positive signals were in the spleen, liver, and kidney. The PCR assay of ALV-J gp85 confirmed 545 base pairs with only 43 positive samples (75.4%). Two positive samples were sequenced and submitted to the Genbank with accession numbers (OR509852-OR509853). Phylogenetic analysis based on the gp85 gene showed that the ALV-J Dakahlia-2 isolate is genetically related to ALV-EGY/YA 2021.3, ALV-EGY/YA 2021.4, ALV-EGY/YA 2021.14, and ALV-EGY/YA 2021.9 with amino acid identity percentage 96%, 97%; 96%, 96%; respectively. Furthermore, ALV-J Sharqia-1 isolate is highly genetically correlated to ALV-EGY/YA 2021.14, and ALV-EGY/YA 2021.9, ALV-J isolate QL1, ALV-J isolate QL4, ALV-J isolate QL3, ALV-EGY/YA 2021.4 with amino acid identity percentage 97%, 97%; 98%, 97%, 97%, 95%; respectively.
conclusionsThis study confirmed that ALV-J infection had still been prevalent in broilers in Egypt, and the genetic characteristics of the isolates are diverse.
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